ASM10568-A680-100 | FNIP1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FNIP1 Polyclonal Antibody (ASM10568). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (ASM10568) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FNIP1 Antibody (D) Composite.
Western blot analysis of Mouse, Rat Kidney showing detection of ~131 kDa FNIP1 protein using Rabbit Anti-FNIP1 Polyclonal Antibody (ASM10568). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Lane 3: Rat Kidney cell lysates. Load: 20 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FNIP1 Polyclonal Antibody (ASM10568) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~131 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q8TF40
Other Accession	NP_001008738.2
Host	Rabbit
Reactivity	Mouse, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human FNIP1 Polyclonal
Target/Specificity	Detects ~130 kDa.
Other Names	FNIP1 Antibody, Folliculin-interacting protein 1 Antibody, Folliculin interacting protein 1 Antibody, KIAA1961 Antibody
Immunogen	Synthetic peptide from the mid-protein of Human FNIP1
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-718 was sufficient for detection of FNIP1 in 15 µg of mouse kidney cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasm

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