K2222-100 assays | Fructose-6-Phosphate Fluorometric Assay Kit Clinisciences

Background

Fructose-6-phosphate (F6P) is a key intermediate in the glycolytic pathway that leads from glucose to pyruvate. F6P is produced from glucose-6-phosphate and is phosphorylated to fructose-1,6-diphosphate which is then cleaved to dihydroxyacetone phosphate and glyceraldehyde phosphate. The transformation of glucose-6-phosphate is regulated by phosphoglucose isomerase, a very interesting enzyme which acts as an isomerase, an autocrine motility factor, a neuroleukin, and a differentiation and maturation mediator.

The Fructose-6-Phosphate Fluorometric Assay Kit provides a highly sensitive, simple, fast and convenient way for detection of F6P levels in various samples based on fluorometric method. In the assay, F6P is converted to glucose-6-phosphate which is then oxidized with the production of a fluorescent product (Ex/Em 535/587 nm). The kit can detect 0.01 to 0.5 nmoles F6P with detection sensitivity of ~1 µM.

Features & Properties

Features	Fast and convenient; Simple procedure; Takes approx. 15-20 minutes to complete the assay; Sensitive, efficient and accurate assay.
Shipping	Blue Ice
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

F6P Assay Buffer, F6P Probe, F6P Enzyme Mix, F6P Converter, F6P Developer, F6P Standard (10 µmol)

Fructose-6-Phosphate Fluorometric Assay Kit

Cat# K2222-100 assays

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Brand : APExBIO Technology