Goat IGF1 ELISA Kit
Cat# orb782502-48
Size : 48Test
Brand : Biorbyt
Contact local distributor :
Phone : +1 850 650 7790
| Catalog Number | orb782502 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1(IGF1). |
| Tested applications | ELISA |
| Reactivity | Goat |
| Range | 1.57-100 ng/mL |
| Concentration | 200 ng/mL |
| UniProt ID | P51457 |
| Sensitivity | 0.65 ng/mL |
| Protein Length | 3.5h |
| Alternative names | IGFI ELISA Kit, IGF1A ELISA Kit, IBP1 ELISA Kit, M |
| Note | For research use only |
| Application notes | Standard: 100 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |