Grn (NM_008175) Mouse Tagged ORF Clone
Cat# MR225358
Size : 10ug
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| Product Data | |
| Target Symbol | Grn |
|---|---|
| Synonyms | epithelin; Pgrn |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >MR225358 representing NM_008175 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCCAGGGAGCCCGGACCCCGACGCAGGCAGACCATGTGGGTCCTGATGAGCTGGCTGGCCTTCG CGGCAGGGCTGGTAGCCGGAACACAGTGTCCAGATGGGCAGTTCTGCCCTGTTGCCTGCTGCCTTGACCA GGGAGGAGCCAACTACAGCTGCTGTAACCCTCTTCTGGACACATGGCCTAGAATAACGAGCCATCATCTA GATGGCTCCTGCCAGACCCATGGCCACTGTCCTGCTGGCTATTCTTGTCTTCTCACTGTGTCTGGGACTT CCAGCTGCTGCCCGTTCTCTAAGGGTGTGTCTTGTGGTGATGGCTACCACTGCTGCCCCCAGGGCTTCCA CTGTAGTGCAGATGGGAAATCCTGCTTCCAGATGTCAGATAACCCCTTGGGTGCTGTCCAGTGTCCTGGG AGCCAGTTTGAATGTCCTGACTCTGCCACCTGCTGCATTATGGTTGATGGTTCGTGGGGATGTTGTCCCA TGCCCCAGGCCTCTTGCTGTGAAGACAGAGTGCATTGCTGTCCCCATGGGGCCTCCTGTGACCTGGTTCA CACACGATGCGTTTCACCCACGGGCACCCACACCCTACTAAAGAAGTTCCCTGCACAAAAGACCAACAGG GCAGTGTCTTTGCCTTTTTCTGTCGTGTGCCCTGATGCTAAGACCCAGTGTCCCGATGATTCTACCTGCT GTGAGCTACCCACTGGGAAGTATGGCTGCTGTCCAATGCCCAATGCCATCTGCTGTTCCGACCACCTGCA CTGCTGCCCCCAGGACACTGTATGTGACCTGATCCAGAGTAAGTGCCTATCCAAGAACTACACCACGGAT CTCCTGACCAAGCTGCCTGGATACCCAGTGAAGGAGGTGAAGTGCGACATGGAGGTGAGCTGCCCTGAAG GATATACCTGCTGCCGCCTCAACACTGGGGCCTGGGGCTGCTGTCCATTTGCCAAGGCCGTGTGTTGTGA GGATCACATTCATTGCTGCCCGGCAGGGTTTCAGTGTCACACAGAGAAAGGAACCTGCGAAATGGGTATC CTCCAAGTACCCTGGATGAAGAAGGTCATAGCCCCCCTCCGCCTGCCAGACCCACAGATCTTGAAGAGTG ATACACCTTGTGATGACTTCACTAGGTGTCCTACAAACAATACCTGCTGCAAACTCAATTCTGGGGACTG GGGCTGCTGTCCCATCCCAGAGGCTGTCTGCTGCTCAGACAACCAGCATTGCTGCCCTCAGGGCTTCACA TGTCTGGCTCAGGGGTACTGTCAGAAGGGAGACACAATGGTGGCTGGCCTGGAGAAGATACCTGCCCGCC AGACAACCCCGCTCCAAATTGGAGATATCGGTTGTGACCAGCATACCAGCTGCCCAGTAGGGCAAACCTG CTGCCCAAGCCTCAAGGGAAGTTGGGCCTGCTGCCAGCTGCCCCATGCTGTGTGCTGTGAGGACCGGCAG CACTGTTGCCCGGCCGGGTACACCTGCAATGTGAAGGCGAGGACCTGTGAGAAGGATGTCGATTTTATCC AGCCTCCCGTGCTCCTGACCCTCGGCCCTAAGGTTGGGAATGTGGAGTGTGGAGAAGGGCATTTCTGCCA TGATAACCAGACCTGTTGTAAAGACAGTGCAGGAGTCTGGGCCTGCTGTCCCTACCTAAAGGGTGTCTGC TGTAGAGATGGACGTCACTGTTGCCCCGGTGGCTTCCACTGTTCAGCCAGGGGAACCAAGTGTTTGCGAA AGAAGATTCCTCGCTGGGACATGTTTTTGAGGGATCCGGTCCCAAGACCGCTACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR225358 representing NM_008175 Red=Cloning site Green=Tags(s) MPPREPGPRRRQTMWVLMSWLAFAAGLVAGTQCPDGQFCPVACCLDQGGANYSCCNPLLDTWPRITSHHL DGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLGAVQCPG SQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNR AVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTD LLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGI LQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFT CLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQ HCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVC CRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDPVPRPLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_008175 |
| ORF Size | 1806 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008175.4, NP_032201.2 |
| RefSeq Size | 2348 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 14824 |
| UniProt ID | P28798 |
| Cytogenetics | 11 66.29 cM |
| MW | 65.5 kDa |
| Summary | Secreted proteins that act as key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28073925, PubMed:8496151, PubMed:28541286, PubMed:28453791, PubMed:20026663, PubMed:23041626, PubMed:27789271, PubMed:12524533). Functions as regulator of proteins trafficking to lysosome and activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286, PubMed:27789271). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structure (PubMed:12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:8496151). Moreover, modulates inflammation in neuron by preserving neuron survival, axonal outgrowth and neuronal integrity (PubMed:23041626, PubMed:20026663).[UniProtKB/Swiss-Prot Function] |
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