HEL 92.1.7
Brand : CLS Cell Lines Service
HEL 92.1.7
General information
Description | The HEL 92.1.7 cell line exhibits the capacity for spontaneous differentiation into erythroblast-like cells, mimicking some aspects of erythroid maturation in vitro. This characteristic makes them particularly useful for studying the erythroid differentiation process and the regulation of gene expression related to erythropoiesis. Their ability to spontaneously differentiate offers a unique advantage for studying the intrinsic pathways and mechanisms that drive erythroid precursor maturation without the addition of external differentiation-inducing agents. Moreover, the differentiation of HEL 92.1.7 cells can be further manipulated through the addition of phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid), which are known to induce macrophage-like differentiation. This induced differentiation into macrophage-like cells expands the utility of the HEL 92.1.7 cell line beyond erythroid studies, allowing researchers to explore and understand the plasticity of hematopoietic cells and the conditions under which lineage commitment and cellular identity can be redirected. Such studies are crucial for developing therapeutic strategies aimed at manipulating cell fate for regenerative medicine and cancer treatment. |
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Organism | Human |
Tissue | Bone marrow |
Disease | Erythroleukemia |
Synonyms | HEL92.1.7, HEL-92.1.7, HEL-92-1-7, HEL-92_1_7, HEL-92, HEL92 |
Characteristics
Age | 30 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | Erythroblast |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | HEL 92.1.7 (Cytion catalog number 300462) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | HLA A3, Aw32, Bw35, Ia+ |
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Products | Hemoglobin, globin (G gamma, A gamma, epsilon, zeta and alpha chains), beta-2-microglobulin, glycophorin |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 10 D13S317: 9,11 D16S539: 11 D5S818: 11 D7S820: 7 TH01: 7 TPOX: 11 vWA: 14,17 D3S1358: 15 D21S11: 29,30.2 D18S51: 12,16 Penta E: 13,18 Penta D: 11,13 D8S1179: 13,15 FGA: 22,23 |
HLA alleles | A*: 03:01:01, 32:01:01 B*: 35:01:01, 35:08:01 C*: 04:01:01 DRB1*: 07:01:01, 13:03:01 DQA1*: 02:01:01, 05:05:01 DQB1*: 02:02:01, 03:01:01 DPB1*: 02:01:02, 04:01:01 E: 01:01:01, 01:03:02 |