Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl₂, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.

 Includes for 500 U:
– 100 μL Horse-Power™ Taq Polymerase (5 U/μL)
– 25 mM MgCl₂ (1.5 mL)
– 1.5 mL Buffer (10x)

• Routine amplifications.
• Colony screening (see Horse-Power™ Red-Taq DNA Polymerase).
• Amplifications up to 5 kb using plasmid, viral or genomic DNA as template.
• PCR fragments amplification for TA or GC cloning (preferably use a proofreading polymerase for cloning purposes combined with an efficient blunt cloning vector) (see pSpark® DNA Cloning System).

t

• Functionally tested in PCR.
• Undetected bacterial DNA (by PCR).
• Undetectable nucleases (endo-, exo, and ribonucleases) activities guaranteed by appropriate quality tests.

• Shipped in: Gel pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

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• Ortiz‐Bustos, C. M., López‐Bernal, A., Testi, L., & Molinero‐Ruiz, L. Environmental and irrigation conditions can mask the effect of Magnaporthiopsis maydis on growth and productivity of maize. Plant Pathology.
• SERRA MARTÍNEZ,. (2017). Detección de posibles fuentes de inoculo de Fusicladium eriobotryae en níspero.
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• Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 127(3), 323-340.
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• Negreiros, A. M. P., Sales Júnior, R., León, M., Melo, N. J. D. A., Michereff, S. J., Ambrósio, M. M. D. Q., … & Armengol, J. Identification and pathogenicity of Macrophomina species collected from weeds in melon fields in Northeastern Brazil. Journal of Phytopathology.
• Rêgo, T. J. S., Elena, G., Correia, K. C., Tovar‐Pedraza, J. M., Câmara, M. P. S., Armengol, J., … & Berbegal, M. Genetic diversity and population structure of Lasiodiplodia theobromae from different hosts in northeastern Brazil and Mexico. Plant Pathology.
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• Mora-Sala, B., Cabral, A., León, M., Agustí-Brisach, C., Armengol, J., & Abad-Campos, P. Survey, Identification and Characterization of cylindrocarpon-like asexual morphs in Spanish forest nurseries. Plant Disease, (ja).
• Robledo-Mahón, T., Aranda, E., Pesciaroli, C., Rodríguez-Calvo, A., Silva-Castro, G. A., González-López, J., & Calvo, C. (2018). Effect of semi-permeable cover system on the bacterial diversity during sewage sludge composting. Journal of Environmental Management, 215, 57-67.
• Ruiz-Ruano, F. J., Castillo-Martínez, J., Cabrero, J., Gómez, R., Camacho, J. P. M., & López-León, M. D. (2018). High-throughput analysis of satellite DNA in the grasshopper Pyrgomorpha conica reveals abundance of homologous and heterologous higher-order repeats. Chromosoma, 1-18.
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• Ruiz-Estévez, M., Bakkali, M., Martín-Blázquez, R., & Garrido-Ramos, M. A. (2017). Identification and Characterization of TALE Homeobox Genes in the Endangered Fern Vandenboschia speciosa. Genes, 8(10), 275.
• Navarro-Domínguez, B., Ruiz-Ruano, F. J., Camacho, J. P. M., Cabrero, J., & López-León, M. D. (2017). Transcription of a B chromosome CAP-G pseudogene does not influence normal Condensin Complex genes in a grasshopper. Scientific reports, 7(1), 17650.
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• León-Ortega, M., & González-Wangüemert, M. (2015). Characterization of 10 new tetranucleotide microsatellite markers for the European eagle owl, Bubo bubo: Useful tools for conservation strategies. Biochemical Systematics and Ecology, 63, 109-112.
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• Jiménez-Hidalgo, M., Kurz, C. L., Pedrajas, J. R., Naranjo-Galindo, F. J., González-Barrios, M., Cabello, J., … & Fierro-González, J. C. (2014). Functional characterization of thioredoxin 3 (TRX-3), a Caenorhabditis elegans intestine-specific thioredoxin. Free Radical Biology and Medicine, 68, 205-219.
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• García-Carneros, A. B., García-Ruiz, R., & Molinero-Ruiz, L. (2014). Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower. Helia, 37(61), 205-214.
• Ortiz-Bustos, C. M., Testi, L., García-Carneros, A. B., & Molinero-Ruiz, L. (2016). Geographic distribution and aggressiveness of Harpophora maydis in the Iberian peninsula, and thermal detection of maize late wilt. European Journal of Plant Pathology, 144(2), 383-397.
• Soto, E. C., Molina-Alcaide, E., Khelil, H., & Yáñez-Ruiz, D. R. (2013). Ruminal microbiota developing in different in vitro simulation systems inoculated with goats’ rumen liquor. Animal Feed Science and Technology, 185(1), 9-18.
• Rodríguez-Ruano, S. M., Martín-Vivaldi, M., Martín-Platero, A. M., López-López, J. P., Peralta-Sánchez, J. M., Ruiz-Rodríguez, M., … & Martínez-Bueno, M. (2015). The Hoopoe’s uropygial gland hosts a bacterial community influenced by the living conditions of the bird. PloS one, 10(10), e0139734.
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This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.