Human HDAC5 (Histone Deacetylase 5) Sandwich ELISA Kit

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	HDAC5
Gene ID:	10014
Uniprot ID:	HDAC5_HUMAN
Sample Type:	tissue homogenates or other biological fluids.

Tissue Specificity	Ubiquitous.
Post Translational Modifications	Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
Protein Name	Histone Deacetylase 5 Hd5 Antigen Ny-Co-9
Database Links	Reactome: R-HSA-2122947 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-350054 Reactome: R-HSA-8943724
Cellular Localisation	Nucleus Cytoplasm Shuttles Between The Nucleus And The Cytoplasm In Muscle Cells It Shuttles Into The Cytoplasm During Myocyte Differentiation The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-259 And Ser-498 By Ampk Camk1 And Sik1
Alternative ELISA Names	Histone Deacetylase 5 ELISA kit Hd5 ELISA kit Antigen Ny-Co-9 ELISA kit HDAC5 ELISA kit KIAA0600 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance