K1031-100 | HyperPFU™ high-fidelity DNA polymerase Clinisciences

Product Citation

1. Qing Wang, Zhiyi He, et al. "Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice." BMC Biotechnol. 2024 Mar 8;24(1):13. PMID: 38459479
2. Ruoxia Wu, Jiaqing Xiong, et al. "Quercetin/Anti-PD-1 Antibody Combination Therapy Regulates the Gut Microbiota, Impacts Macrophage Immunity and Reshapes the Hepatocellular Carcinoma Tumor Microenvironment." Front Biosci (Landmark Ed). 2023 Dec 1;28(12):327. PMID: 38179731
3. Pablo Rodríguez-Fernández, Laure Botella, et al. "MptpB Inhibitor Improves the Action of Antibiotics against Mycobacterium tuberculosis and Nontuberculous Mycobacterium avium Infections." ACS Infect Dis. 2023 Dec 12. PMID: 38085851
4. Wen Sheng, Wenjing Xu, et al. "Guijiajiao (Colla Carapacis et Plastri, CCP) prevents male infertility via gut microbiota modulation." Chin J Nat Med. 2023 Jun;21(6):403-410. PMID: 37407171
5. Man Luo, Yuanyuan Chen, et al. "E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism." Front Immunol. 2023 May 19:14:1137089. PMID: 37275915
6. Li Zhang, Junjuan Lu, et al. "Akkermansia muciniphilaAmeliorates Lung Injury in Smoke-Induced COPD Mice by IL-17 and Autophagy." Cellular Microbiology. 15 March 2023.
7. Yao B, Cai Y, et al. "The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration." Orthop Surg 2023 Jan 04. PMID: 36600636

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Description

HyperPFU™ high-fidelity DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, it produces PCR products with very high speed and accuracy. Also, HyperPFU™ high-fidelity DNA polymerase is tolerant of various inhibitors, allowing for robust amplification with minimal optimization. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. It becomes the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
HyperPFU™ high-fidelity DNA polymerase is important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), it is one of the most accurate thermostable polymerases available. It possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
HyperPFU™ high-fidelity DNA polymerases is supplied with standard 5X HF Buffer, which can be used for complex or GC-rich templates.

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HyperPFU™ high-fidelity DNA polymerase

Cat# K1031-100

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Brand : APExBIO Technology

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Description

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