IL7 Receptor alpha antibody [A7R34] (PerCP-Cy5.5)
Cat# GTX01473-11-100ug
Size : 100ug
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-IL7 Receptor alpha antibody [A7R34] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01473-11](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-IL7 Receptor alpha antibody [A7R34] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01473-11")
Host | Rat |
|---|---|
Clonality | Monoclonal |
Clone Name | A7R34 |
Isotype | IgG2a |
Application | FACS |
Reactivity | Human, Mouse |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX01473-11 on CiteAb
PRODUCT
Summary
The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | Assay dependent |
Not tested in other applications.
Calculated MW
52 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 7 receptor , CD127 , IL-7Ralpha
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
| | GTX01473-11 FACS Image FACS analysis of mouse C57Bl/6 splenocytes using GTX01473-11 IL7 Receptor alpha antibody [A7R34] (PerCP-Cy5.5). |
Clone Reference
2018; STAT3 regulates cytotoxicity of human CD57+ CD4+ T cells in blood and lymphoid follicles.
Application : FACS
Reactivity : Human
2012; Activation of the Hippo pathway by CTLA-4 regulates the expression of Blimp-1 in the CD8+ T cell.
2011; In vivo administration of the recombinant IL-7/hepatocyte growth factor β hybrid cytokine efficiently restores thymopoiesis and naive T cell generation in lethally irradiated mice after syngeneic bone marrow transplantation.
2009; LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.
2006; Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.
Submit a Reference REVIEW
Clone Reference
2018; STAT3 regulates cytotoxicity of human CD57+ CD4+ T cells in blood and lymphoid follicles.
Application : FACS
Reactivity : Human
2012; Activation of the Hippo pathway by CTLA-4 regulates the expression of Blimp-1 in the CD8+ T cell.
2011; In vivo administration of the recombinant IL-7/hepatocyte growth factor β hybrid cytokine efficiently restores thymopoiesis and naive T cell generation in lethally irradiated mice after syngeneic bone marrow transplantation.
2009; LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.
2006; Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.
2002; TCR signals mediated by Src family kinases are essential for the survival of naive T cells.
1993; Expression and function of the interleukin 7 receptor in murine lymphocytes.
| SDS | |
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| Sodium Azide.pdf | |