Interferon alpha (IFN alpha) Rabbit Polyclonal Antibody
Cat# SP2126A
Size : 500ug
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Interferon alpha (IFN alpha) Rabbit Polyclonal Antibody
| Product Data | |
| Application | ELISA, FN, IP, WB |
|---|---|
| Recommended Dilution | ELISA. Western Blot: This antibody cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma; this antibdoy detects a band of approximately 19kDa. Functional Assays. Immunoprecipitation. |
| Reactivity | Chicken |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Recombinant chicken Interferon alpha. |
| Specificity | This antibody recognizes Interferon alpha (chIFN-alpha). In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities. Product SA027 is the immunogen used to produce this antibody. |
| Buffer | PBS State: Azide Free State: Liquid purified IgG Stabilizer: None Preservative: None |
| Concentration | lot specific |
| Purification | Affinity chromatography on protein G |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Background | The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent. |
| Synonyms | B Cell Interferon, IFN-alpha, IFNA, Interferon-alpha, Leukocyte Interferon, Type I Interferon |
| Reference Data | |