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QC Test

The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

  • Specifications

    Product Description

    Iron Assay Kit is a quantitative colorimetric iron determination at 590 nm.This kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590 nm, is directly proportional to the iron concentration in the sample.

    Suitable Sample

    Biological (e.g. serum) and environmental samples.

    Sample Volume

    50 uL (using 96-well plate)
    250 uL (using cuvette)

    Detection Method

    Colorimetric

    Assay Type

    Quantitative

    Calibration Range

    27 ug/dL (4.8 uM) to 1,000 ug/dL (179 uM) in 96-well plate assay.

    Regulation Status

    For research use only (RUO)

    Quality Control Testing

    Standard Curve

    The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

    Storage Instruction

    Store reagent A at room temperature and other reagents at 4°C.

  • Applications

    Functional Study

  • Publication Reference