Specifications

Product Data
Applications	ELISA, ID, IF, IP, WB
Recommended Dilution	Can be used in enzyme-immunocytochemical and immunohistochemical techniques for the indirect detection of human lactoferrin at the cellular and subcellular level in appropriately treated cell and tissue substrates; as catching reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). The optimum working dilution of the product should be established by titration before being used. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/500.
Reactivities	Human
Host	Goat
Isotype	IgG
Clonality	Polyclonal
Immunogen	Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. Cross-reactivity:The antiserum does not cross-react with any other human plasma proteins as tested in geldiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.
Formulation	Phosphate buffered saline, pH 7.2 - No preservative added. State: Azide Free State: Lyphilized purified IgG fraction
Reconstitution Method	Restore with 1,0 ml sterile distilled water
Concentration	lot specific
Purification	Fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography
Conjugation	Unconjugated
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	lactotransferrin
Database Link	Entrez Gene 4057 Human UniProt ID P02788
Synonyms	LTF, LF, Lactoferrin, EC=3.4.21, Talalactoferrin
Note	Adsorption: Immunoaffinity adsorbed using insolubilized fractions of human serum and lactoferrin– depleted human milk as required, to eliminate antibodies reacting with other human serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Reference Data
Protein Families	Druggable Genome, Protease, Secreted Protein