Reagents and instruments for immunology, cell biology and molecular biology.
 
> LC3 Antibody (APG8B) (N-term)

LC3 Antibody (APG8B) (N-term)

Print Print
Please log in

Cat# AP1802a

Size : 200ul

Brand : Abcepta

Request more information

Contact local distributor :


Phone : +1 850 650 7790

 
  • IF - LC3 Antibody (APG8B) (N-term) AP1802A
    Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term)(Cat. #AP1802a). U251 cells(right) were treated with Chloroquine (50 μM,16h). AP1802a was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
  • WB - LC3 Antibody (APG8B) (N-term) AP1802A
    Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (N-term)(Cat. #AP1802a)(upper) or Beta-actin (lower).
  • WB - LC3 Antibody (APG8B) (N-term) AP1802A
    Western blot analysis of lysates from NIH/3T3, HT-1080 cell line, untreated or treated with chloroquine, 50μM, using LC3 Antibody (APG8B) (Cat. #AP1802a)(upper) or GAPDH(lower).
  • IHC-P - LC3 Antibody (APG8B) (N-term) AP1802A
    Wild-type (Cln3+/+) or homozygous Cln3Äex7/8 (Cln3Äex7/8/Äex7/8) paraffin-embedded brain sections immunostained for the LC3 protein (Cat. # AP1802a LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3Äex7/8 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer. Data courtesy of Dr. Susan Cotman, Massachusets General Hospital.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9GZQ8
Other Accession A6NCE7, O41515, Q9CQV6
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14688 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 81631
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
Target/Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Dilution IF~~1:100
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLC3 Antibody (APG8B) (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3B (HGNC:13352)
Synonyms MAP1ALC3
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed:30420355).
Cellular Location Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor Endomembrane system; Lipid-anchor Mitochondrion membrane; Lipid-anchor. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q9CQV6}. Cytoplasmic vesicle. Note=LC3-II binds to the autophagic membranes. LC3-II localizes with the mitochondrial inner membrane during Parkin-mediated mitophagy (PubMed:28017329). Localizes also to discrete punctae along the ciliary axoneme
Tissue Location Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT