Reagents and instruments for immunology, cell biology and molecular biology.

LNCaP

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Cat# 300265

Size : 1cryovial

Contact local distributor :

Phone : +1 850 650 7790

Brand : CLS Cell Lines Service

Contact local distributor :

Phone : +1 850 650 7790

LNCaP Cells

Description	LNCaP cells, derived from a metastatic lesion in a lymph node of a prostate cancer patient, represent a critical tool in prostate cancer research, particularly for studying the role of androgens and androgen receptor (AR) dynamics in cancer progression. The LNCaP cell line is characterized by their androgen-sensitive growth and offers a window into the mechanisms underlying prostate cancer's response to hormonal manipulation. As a model for metastatic prostate cancer, parental LNCaP cells and their sublines, such as the LNCaP clone FGC, provide clinically relevant insights into disease progression, especially in the context of metastasis to bone, forming osteoblastic lesions akin to those observed in human prostate cancer. The LNCaP human prostate cancer cell line expresses a mutated form of the AR gene with broader steroid-binding specificity and therefore is pivotal for understanding the complex interplay between AR activity and prostate cancer progression. This includes the examination of AR downstream targets like PSA and NKx3.1, which are crucial for prostatic epithelial cell function. LNCaP cells are further used in cytotoxicity studies such as those induced by ripl or the potential therapeutic effects of compounds like amygdalin, within the scope of intracellular drug delivery strategies. In summary, the human prostate carcinoma cell line LNCaP serves as a cornerstone in understanding the role of androgens in cancer progression and prostate cancer, offering insights into hormone-responsive cancers, the challenges of resistant prostate cancer, and the potential for therapeutic interventions. The LNCaP cell line is considered one of the classic and most widely used human prostate cancer cell lines, alongside DU145 and PC3 cells.
Organism	Human
Tissue	Prostate
Disease	Carcinoma
Metastatic site	Left supraclavicular lymph node
Synonyms	LNCAP, LNCap, Ln-Cap, Lymph Node Carcinoma of the prostate

Age	50 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent, clusters

Citation	LNCaP (Cytion catalog number 300265)
Biosafety level	1

Receptors expressed	Androgen, estrogen
Protein expression	p53 positive
Tumorigenic	Yes, in nude mice
Products	human prostatic acid phosphatase, prostate specific antigen
Karyotype	pseudodiploid male, seven marker chromosomes, modal number = 46, range = 33 to 91

Culture Medium	EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c)
Medium supplements	Supplement the medium with 10% FBS
Passaging solution	Accutase
Doubling time	60 hours
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	A ratio of 1:3 to 1:6 is recommended
Seeding density	1 to 2 x 10^4 cells/cm^2
Fluid renewal	Every 3 days
Freezing recovery	After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100)
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Optionally, skip centrifugation but remove any remaining freezing medium after 24 hours. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
STR profile	Amelogenin: x,y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11 D5S818: 11,12 D7S820: 9.1,10.3 TH01: 9 TPOX: 8,9 vWA: 16,18 D3S1358: 16 D21S11: 29,31.2 D18S51: 11,12 Penta E: 12,16 Penta D: 12,12.4 D8S1179: 12,14 FGA: 19,20
HLA alleles	A: 01:01:01, 02:01:01 B: 08:01:01, 37:01:01 C: 06:02:01, 07:01:01 DRB1: 03:01:01, 10:01:01 DQA1: 01:05:01, 05:01:01 DQB1: 02:01:01, 05:01:01 DPB1*: 02:01:02G, 04:02:01G E: 01:01:01

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LNCaP

Cat# 300265

Contact local distributor :

Brand : CLS Cell Lines Service

Contact local distributor :

LNCaP Cells

Key points about the LNCaP cell line

Properties of the prostate cancer cell line LNCaP

Documentation

Genotype

Handling the androgen-sensitive LNCaP cell line

Quality control

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