- More Files
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: LRP6-hIgGFc transfected HEK293 cell lysates with LRP6 monoclonal antibody, clone 1A3F5 (Cat # MAB21388).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with LRP6 monoclonal antibody, clone 1A3F5 (Cat # MAB21388).
Flow Cytometry
Flow cytometric analysis of HL-60 cells with LRP6 monoclonal antibody, clone 1A3F5 (Cat # MAB21388) (Green). Red: Negative Control.
- Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant human LRP6.
Immunogen
Recombinant protein corresponding to amino acids 1198-1370 of human LRP6.
Host
Mouse
Theoretical MW (kDa)
180
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: LRP6-hIgGFc transfected HEK293 cell lysates with LRP6 monoclonal antibody, clone 1A3F5 (Cat # MAB21388).Enzyme-linked Immunoabsorbent Assay
ELISA analysis with LRP6 monoclonal antibody, clone 1A3F5 (Cat # MAB21388).Flow Cytometry
Flow cytometric analysis of HL-60 cells with LRP6 monoclonal antibody, clone 1A3F5 (Cat # MAB21388) (Green). Red: Negative Control. - Gene Info — LRP6
Entrez GeneID
4040Protein Accession#
O75581Gene Name
LRP6
Gene Alias
ADCAD2, FLJ90062, FLJ90421
Gene Description
low density lipoprotein receptor-related protein 6
Gene Ontology
HyperlinkGene Summary
The LRP6 gene encodes a member of the low density lipoprotein receptor (LDLR) gene family, which consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDLR proteins are comprised of the same basic structural motifs: an extracellular domain that contains LDLR binding repeats and EGF repeats with associated spacer domains containing the YWTD motif; a single transmembrane-spanning domain; and a C-terminal cytoplasmic domain that generally contains at least 1 copy of the NPXY motif (Brown et al., 1998 [PubMed 9704021]). LRP6 and LRP5 (MIM 603506) function as coreceptors for WNT (MIM 164820) ligands and thus play a role in WNT signaling, which is important in a wide variety of biologic processes during pre- and postnatal life in invertebrates and vertebrates (Kokubu et al., 2004 [PubMed 15469977]).[supplied by OMIM
Other Designations
low density lipoprotein-related protein 6
- Interactomes
- Pathways
- Diseases