MAPK1 Positive Control

Formulation:	Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range:	WB: 1:500
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MAPK1
Gene ID:	5594
Uniprot ID:	MK01_HUMAN
Specificity:	This is positive control is recommended for use in combination with MAPK1 antibody STJ501679.

Post Translational Modifications	Phosphorylated upon KIT and FLT3 signaling. Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. Dephosphorylated by DUSP1 and DUSP2 at Thr-185 and Tyr-187 . ISGylated. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination.leading to activation. Deubiquitinated by CYLD.
Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2. Acts as a transcriptional repressor. Binds to a GCAAAGC consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Peptide Name	Mitogen-Activated Protein Kinase 1 Map Kinase 1 Mapk 1 Ert1 Extracellular Signal-Regulated Kinase 2 Erk-2 Map Kinase Isoform P42 P42-Mapk Mitogen-Activated Protein Kinase 2 Map Kinase 2 Mapk 2
Database Links	Reactome: R-HSA-111995 Reactome: R-HSA-112409 Reactome: R-HSA-112411 Reactome: R-HSA-1181150 Reactome: R-HSA-1295596 Reactome: R-HSA-1502540 Reactome: R-HSA-162658 Reactome: R-HSA-170968 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2029482 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559585 Reactome: R-HSA-2871796 Reactome: R-HSA-3371453 Reactome: R-HSA-375165 Reactome: R-HSA-437239 Reactome: R-HSA-444257 Reactome: R-HSA-445144 Reactome: R-HSA-450341 Reactome: R-HSA-456926 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5663213 Reactome: R-HSA-5668599 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6798695 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-6811558 Reactome: R-HSA-74749 Reactome: R-HSA-877300 Reactome: R-HSA-879415 Reactome: R-HSA-881907 Reactome: R-HSA-8939211 Reactome: R-HSA-8940973 Reactome: R-HSA-8943724 Reactome: R-HSA-9627069 Reactome: R-HSA-9634635 Reactome: R-HSA-9634638 Reactome: R-HSA-9635465 Reactome: R-HSA-9649948 Reactome: R-HSA-9652169 Reactome: R-HSA-9652817 Reactome: R-HSA-9656223 Reactome: R-HSA-9664422 Reactome: R-HSA-9725371 Reactome: R-HSA-9732724 Reactome: R-HSA-9768919 Reactome: R-HSA-982772
Cellular Localisation	Cytoplasm Cytoskeleton Spindle Nucleus Microtubule Organizing Center Centrosome Membrane Caveola Cell Junction Focal Adhesion Associated With The Spindle During Prometaphase And Metaphase Pea15-Binding And Phosphorylated Dapk1 Promote Its Cytoplasmic Retention Phosphorylation At Ser- 246 And Ser-248 As Well As Autophosphorylation At Thr-190 Promote Nuclear Localization
Alternative Peptide Names	Mitogen-Activated Protein Kinase 1 protein Map Kinase 1 protein Mapk 1 protein Ert1 protein Extracellular Signal-Regulated Kinase 2 protein Erk-2 protein Map Kinase Isoform P42 protein P42-Mapk protein Mitogen-Activated Protein Kinase 2 protein Map Kinase 2 protein Mapk 2 protein MAPK1 protein ERK2 protein PRKM1 protein PRKM2 protein

Information sourced from Uniprot.org

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