Mirabegron [223673-61-8]
Cat# HY-14773-50mg
Size : 50mg
Brand : MedChemExpress
Mirabegron is a selective β3-adrenoceptor agonist with EC50 of 22.4 nM.
For research use only. We do not sell to patients.
Mirabegron Chemical Structure
CAS No. : 223673-61-8
This product is a controlled substance and not for sale in your territory.
Based on 5 publication(s) in Google Scholar
Other Forms of Mirabegron:
- (Rac)-Mirabegron-d5 Get quote
- Mirabegron (Standard) Get quote
View All Adrenergic Receptor Isoform Specific Products:
Description |
Mirabegron is a selective β3-adrenoceptor agonist with EC50 of 22.4 nM. |
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IC50 & Target |
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In Vitro |
Mirabegron (YM178) increases cyclic AMP accumulation in Chinese hamster ovary (CHO) cells expressing human β3-adrenoceptor (AR). EC50 value is 22.4 nM. EC50 values of Mirabegron for human β1- and β2-ARs are 10,000 nM or more, respectively. EC50 of Mirabegron in rat bladder strips precontracted with 10-6 M Carbachol (CCh) is 5.1 μM, whereas that in human bladder strips precontracted with 10-7 M CCh is 0.78 μM. Mirabegron concentration-dependently increases the accumulation of cAMP in CHO cells expressing human β3-ARs, with an EC50 value and I.A. of 22.4 nM and 0.8, respectively. Mirabegron has little agonistic effect on β1- and β2-ARs. Compared by EC50 value, Mirabegron is approximately one third as potent as isoproterenol. The maximal relaxant effects of Mirabegron are 94±1%, that of CCh, indicating that Mirabegron acts a full agonist in the rat bladder. The maximal relaxant effects of Mirabegron is 89.4±2.3%[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
Mirabegron (YM178) produces a dose-dependent decrease in the frequency of rhythmic bladder contraction in anesthetized rats. In contrast, Mirabegron does not decrease the amplitude of rhythmic bladder contraction at up to 3 mg/kg i.v.. On the contrary, Oxybutynin significantly increases the frequency of rhythmic bladder contraction and decreased its amplitude at doses of 0.272 mg/kg i.v. or more[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Clinical Trial |
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Molecular Weight |
396.51 |
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Formula |
C21H24N4O2S |
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CAS No. |
223673-61-8 |
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Appearance |
Solid |
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Color |
White to light yellow |
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SMILES |
O=C(NC1=CC=C(CCNC[C@H](O)C2=CC=CC=C2)C=C1)CC3=CSC(N)=N3 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : 100 mg/mL (252.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.90% |
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References |
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Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.5220 mL | 12.6100 mL | 25.2200 mL | 63.0501 mL |
5 mM | 0.5044 mL | 2.5220 mL | 5.0440 mL | 12.6100 mL | |
10 mM | 0.2522 mL | 1.2610 mL | 2.5220 mL | 6.3050 mL | |
15 mM | 0.1681 mL | 0.8407 mL | 1.6813 mL | 4.2033 mL | |
20 mM | 0.1261 mL | 0.6305 mL | 1.2610 mL | 3.1525 mL | |
25 mM | 0.1009 mL | 0.5044 mL | 1.0088 mL | 2.5220 mL | |
30 mM | 0.0841 mL | 0.4203 mL | 0.8407 mL | 2.1017 mL | |
40 mM | 0.0631 mL | 0.3153 mL | 0.6305 mL | 1.5763 mL | |
50 mM | 0.0504 mL | 0.2522 mL | 0.5044 mL | 1.2610 mL | |
60 mM | 0.0420 mL | 0.2102 mL | 0.4203 mL | 1.0508 mL | |
80 mM | 0.0315 mL | 0.1576 mL | 0.3153 mL | 0.7881 mL | |
100 mM | 0.0252 mL | 0.1261 mL | 0.2522 mL | 0.6305 mL |
Mirabegron Related Classifications
- GPCR/G Protein Neuronal Signaling
- Adrenergic Receptor