Mouse Anti-Chicken CD8α-UNLB

Cat# 8390-01

Size : 0.5mg

Brand : Southern Biotech

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Phone : +1 850 650 7790

More Information
Clone EP72
Isotype Mouse (BALB/c) IgG2bκ
Isotype Control Mouse IgG2b-UNLB (A-1)
Specificity Chicken CD8α
Description In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two α chains and (ii) a heterodimer of an α chain and a β chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8α- and CD8β-chains, a relatively large proportion of the CD8+ TCRγδ cells in the spleens of embryos and young chicks express only the α-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRγδ CD8αβ cells in the chicken that is not found in rodents. The monoclonal antibody EP72 recognizes the CD8α chain.
Immunogen Chicken splenocytes
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 4-7
Immunohistochemistry-Frozen Sections – Reported in literature 2,3

RRID Number AB_2796537
Gene ID 403158 (Chicken)
Gene ID Symbol CD8A (Chicken)
Gene ID Aliases CD8
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  1. 1. Paramithiotis E, Tkalec L, Ratcliffe MJ. High levels of CD45 are coordinately expressed with CD4 and CD8 on avian thymocytes. J Immunol. 1991;147:3710-7. (Immunogen)
  2. 2. Reemers SS, van Haarlem D, Groot Koerkamp MJ, Vervelde L. Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow. J Gen Virol. 2009;90:2134-46. (IHC-FS)
  3. 3. Reemers SS, Jansen C, Groot Koerkamp MJ, van Haarlem D, van de Haar P, Degen WG, et al. Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: a transcriptomics analysis of adjuvanted vaccines. Vaccine. 2010;28:6351-60. (IHC-FS)
  4. 4. Marmor MD, Benatar T, Ratcliffe MJ. Retroviral transformation in vitro of chicken T cells expressing either α/β or γ/δ T cell receptors by reticuloendotheliosis virus strain T. J Exp Med. 1993;177:647-56. (FC)
  5. 5. Choi KD, Lillehoj HS, Song KD, Han JY. Molecular and functional characterization of chicken IL-15. Dev Comp Immunol. 1999;23:165-77. (FC)
  6. 6. Chen KL, Tsay SM, Chiou PW, Sun CP, Weng BC. Effects of caponization and different forms of exogenous androgen implantation on immunity in male chicks. Poult Sci. 2010;89:887-94. (FC)
  7. 7. Norup LR, Dalgaard TS, Pedersen AR, Juul-Madsen HR. Assessment of Newcastle disease-specific T cell proliferation in different inbred MHC chicken lines. Scand J Immunol. 2011;74:23-30. (FC)