General Info
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.101ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This CACNa1S Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
Information
| Gene Symbol: | Cacna1s |
| Gene ID: | 12292 |
| Uniprot ID: | CAC1S_MOUSE |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | |
| Post Translational Modifications | The alpha-1S subunit is found in two isoforms in the skeletal muscle: a minor form of 212 kDa containing the complete amino acid sequence, and a major form of 190 kDa derived from the full-length form by post-translational proteolysis close to Phe-1690. Phosphorylated. Phosphorylation by PKA activates the calcium channel. Both the minor and major forms are phosphorylated in vitro by PKA. Phosphorylation at Ser-1575 is involved in beta-adrenergic-mediated regulation of the channel. |
| Function | Pore-forming, alpha-1S subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle via their interaction with RYR1, which triggers Ca(2+) release from the sarcplasmic reticulum and ultimately results in muscle contraction. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. |
| Protein Name | Voltage-Dependent L-Type Calcium Channel Subunit Alpha-1sCalcium Channel - L Type - Alpha-1 Polypeptide - Isoform 3 - Skeletal MuscleDihydropyridine ReceptorDhprVoltage-Gated Calcium Channel Subunit Alpha Cav1.1 |
| Database Links | |
| Cellular Localisation | Cell MembraneSarcolemmaT-TubuleMulti-Pass Membrane Protein |
| Alternative ELISA Names | Voltage-Dependent L-Type Calcium Channel Subunit Alpha-1s ELISA kitCalcium Channel - L Type - Alpha-1 Polypeptide - Isoform 3 - Skeletal Muscle ELISA kitDihydropyridine Receptor ELISA kitDhpr ELISA kitVoltage-Gated Calcium Channel Subunit Alpha Cav1.1 ELISA kitCacna1s ELISA kitCach1 ELISA kitCach1b ELISA kitCacn1 ELISA kitCacnl1a3 ELISA kit |
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Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance