Mouse IgA (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP
Specifications
Product Data
Recommended Dilution
Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1:6,300 Western Blot: 1:200 - 1:1,000 Immunohistochemistry: 1:100 - 1:500
Reactivity
Mouse
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Mouse IgG, IgA and IgM whole molecules.
Specificity
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. This reagent is suitable for the detection of all mouse immunoglobulin subclasses and chain combinations.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protesase free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: AP State: Liquid (sterile filtered) F(ab')2 fragments. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration
lot specific
Purification
Immunoaffinity chromatography.
Conjugation
AP
Storage
This antibody is stable at 2-8°C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
