Mouse IgA (H+L chain) goat polyclonal antibody, AP
Specifications
Product Data
Recommended Dilution
Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/500-1/2,500. Immunohistochemistry: 1/200-1/1,000.
Reactivity
Mouse
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Mouse IgG, IgA and IgM whole molecules.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by mmunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum. This reagent is suitable for the detection of all Mouse immunoglobulin isotypes, subclasses and chain combinations.
Buffer
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, with 10 mg/ml BSA (IgG and protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: AP State: Liquid (sterile filtered) purified Ig fraction. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration
lot specific
Purification
Immunoaffinity chromatography.
Conjugation
AP
Storage
Store the antibody (undiluted) at 2-8°C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
