Description | MOUSE IgA Kappa (κ) isotype control |
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Conjugate | Unconjugated |
Clonality | Monoclonal |
Application
| FC |
Application Note | FlowCytometry 1:1000-1:5000 |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgA |
Buffer | 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2 |
Species of Origin | Mouse |
Stabilizer | None |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Wet Ice |
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Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Light and heavy chain composition confirmed by RID. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain. |
Storage Condition | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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Cat# ASR2275