Mouse IgG F(ab')2

Cat# ASR2304

Size : 2mg

Brand : Abcepta

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  •  - Mouse IgG F(ab')2  ASR2304
    SDS-PAGE of Mouse IgG F(ab')2 Fragment . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab')2 Fragment . Lane 5: Mouse IgM Kappa Myeloma Protein . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
Product Information
Description MOUSE IgG F(ab')2 fragment
Conjugate Unconjugated
Physical State Lyophilized
Host Isotype IgG F(ab')2
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Mouse
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Mouse IgG F(ab')2 fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(ab’)2.   No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Mouse IgG F(ab')2 fragment is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.