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> Mouse IgM Kappa (κ) isotype Control

Mouse IgM Kappa (κ) isotype Control

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Cat# ASR2274

Size : 1mg

Brand : Abcepta

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  • - Mouse IgM Kappa (κ) isotype Control ASR2274
    SDS-PAGE of Mouse IgM Kappa isotype control . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa isotype control . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab') at 25 kDa; IgM ? at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
Product Information
Description MOUSE IgM Kappa (κ) isotype control
Conjugate Unconjugated
Clonality Monoclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC
Application Note FlowCytometry 1:1000-1:5000
Physical State Liquid (sterile filtered)
Host Isotype IgM
Buffer 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Species of Origin Mouse
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum.  Light and heavy chain composition confirmed by Elisa.
Storage Condition Store vial at 4° C prior to opening.   Mouse IgM Kappa isotype control is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.