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> Mouse Kappa Antibody : PE

Mouse Kappa Antibody : PE

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Cat# OASB00199

Size : 0.1mg

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for Mouse Kappa Antibody : PE (OASB00199)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityMonoclonal
Clone187.1
IsotypeIgG1
HostRat
ConjugationR-PE
ApplicationFC, FLISA
Reconstitution and StorageStore at 2-8C
ImmunogenMPC-11 immunoglobulin
Concentration0.1 mg/mL
SpecificityKappa
CharacterizationTo ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by ELISA and/or flow cytometryto conform to characteristics of a standard reference reagent. Representative data are included in this productinsert.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry:
ELISA:
Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
Alkaline phosphatase conjugate
Horseradish peroxidase conjugate
<=1 ug/106 cells
<=1 ug/106 cells
<=0.2 ug/106 cells
1:500-1:1,000
1:4,000-1:8,000
Application InfoIdentification and enumeration of cells by flow cytometry, Identification and enumeration of cells by immunofluorescence microscopy, Enzyme-Linked-Immunosorbent-Assay (ELISA)
Other Applications DataSince applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds.
Other Applications Image 1 Data Amount Used: 1 ug/106 cells BALB/c splenocytes were double-stained with rat anti-mouse κ-FITC and rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA).
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected.
Gene SymbolIGK
Gene Full NameIg kappa chain C region
Alias Symbolsk, kappa
NCBI Gene Id243469
Description of TargetSummary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the kappa light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments in this cluster are incapable of encoding a protein and are considered pseudogenes.
Uniprot IDP01837

  1. What is the species homology for "Mouse Kappa Antibody : PE (OASB00199)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Mouse Kappa Antibody : PE (OASB00199)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Mouse Kappa Antibody : PE (OASB00199)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse Kappa Antibody : PE (OASB00199)"?

    This target may also be called "k, kappa" in publications.

  5. What is the shipping cost for "Mouse Kappa Antibody : PE (OASB00199)"?

    The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.

  6. What is the guarantee for "Mouse Kappa Antibody : PE (OASB00199)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse Kappa Antibody : PE (OASB00199)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse Kappa Antibody : PE (OASB00199)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "IGK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "IGK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "IGK"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "IGK"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "IGK"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "IGK"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.