NBD-Cl [10199-89-0]
Cat# HY-D0042-1g
Size : 1g
Brand : MedChemExpress
NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.
For research use only. We do not sell to patients.
NBD-Cl Chemical Structure
CAS No. : 10199-89-0
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups[1]. |
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In Vitro |
NBD-Cl (NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
199.55 |
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Formula |
C6H2ClN3O3 |
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CAS No. |
10199-89-0 |
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Appearance |
Solid |
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Color |
Light yellow to yellow |
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Emission (Em) |
515 |
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Excitation (Ex) |
498 |
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SMILES |
O=[N+](C1=CC=C(Cl)C2=NON=C21)[O-] |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvent & Solubility |
In Vitro:
DMSO : ≥ 100 mg/mL (501.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : < 0.1 mg/mL (insoluble) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.14% COA (253 KB) HNMR (246 KB) RP-HPLC (86 KB) Handling Instructions (2659 KB) |
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References |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 5.0113 mL | 25.0564 mL | 50.1128 mL | 125.2819 mL |
5 mM | 1.0023 mL | 5.0113 mL | 10.0226 mL | 25.0564 mL | |
10 mM | 0.5011 mL | 2.5056 mL | 5.0113 mL | 12.5282 mL | |
15 mM | 0.3341 mL | 1.6704 mL | 3.3409 mL | 8.3521 mL | |
20 mM | 0.2506 mL | 1.2528 mL | 2.5056 mL | 6.2641 mL | |
25 mM | 0.2005 mL | 1.0023 mL | 2.0045 mL | 5.0113 mL | |
30 mM | 0.1670 mL | 0.8352 mL | 1.6704 mL | 4.1761 mL | |
40 mM | 0.1253 mL | 0.6264 mL | 1.2528 mL | 3.1320 mL | |
50 mM | 0.1002 mL | 0.5011 mL | 1.0023 mL | 2.5056 mL | |
60 mM | 0.0835 mL | 0.4176 mL | 0.8352 mL | 2.0880 mL | |
80 mM | 0.0626 mL | 0.3132 mL | 0.6264 mL | 1.5660 mL | |
100 mM | 0.0501 mL | 0.2506 mL | 0.5011 mL | 1.2528 mL |
NBD-Cl Related Classifications
- Others
- Others
- Fluorescent Dye
-
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
NBD-Cl10199-89-0NBDFluorescent DyeInhibitorinhibitorinhibit