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> PCNA Polyclonal Antibody

PCNA Polyclonal Antibody

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Cat# E-AB-40514-100

Size : 100uL

Brand : Elabscience

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Verified Samples Verified Samples in WB: Zebrafish, Hela, A431, NIH/3T3, Raw264.7
Dilution WB 1:1000-2000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Zebrafish
Applications WB
Clonality Polyclonal
Immunogen Recombinant human PCNA protein expressed by E.coli
Abbre PCNA
Synonyms Cyclin,   DNA polymerase delta auxiliary protein,   HGCN8729,   MGC8367,   Mutagen-sensitive 209 protein,   OTTHUMP00000030189,   OTTHUMP00000030190,   PCNAR,   Pcna/cyclin,   Polymerase delta accessory protein,   Proliferating ce,   cb16,   etID36690.10,   fa28e03,   fb36g03,  ATLD2
Swissprot
Calculated MW 35 kDa
Observed MW 35 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Nucleus
Concentration 1.5 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer,  Cell Biology,  Epigenetics and Nuclear Signaling,  Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where
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E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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