PPARA ELISA Kit (Dog)

Cat# OKEH06609

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ppara ELISA Kit (Dog) (OKEH06609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppara has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppara is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppara captured in the well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Ppara Microplate96 Wells (12 x 8 Well strips)
Ppara Lyophilized Standard2 x 5 ng
100X Ppara HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity27 pg/mL
SpecificityNatural and recombinant Dog Peroxisome proliferator-activated receptor alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPPARA
Gene Full Nameperoxisome proliferator activated receptor alpha
Alias Symbolsnuclear receptor subfamily 1 group C member 1;peroxisome proliferative activated receptor, alpha;peroxisome proliferator-activated receptor alpha;PPAR alpha.
NCBI Gene Id403654
Protein NameQ95N78
Description of TargetPeroxisome proliferator-activated receptor alpha
Uniprot IDhttps://www.uniprot.org/uniprot/Q95N78
Protein Accession #NP_001003093
Nucleotide Accession #NM_001003093