Recombinant Human IL-18
Cat# B001-5
Size : 25ug
Brand : MBL International
Contact local distributor :
Phone : +1 850 650 7790
Please Note: MBL International will be shutting down its operations effective December 31, 2024. Distribution of MBL products in the United States will be transferred to Cosmo Bio US on January 1st while European Distributors will remain unchanged. For any US inquiries regarding orders or support during this transition, reach out to Cosmo Bio: https://www.cosmobiousa.com/. For Non-US inquiries reach out to MBL in Japan: https://www.mblbio.com/.
B001-5
Cytokine
25 µg
| Target | IL-18 |
|---|---|
| Product Type | Cytokine |
| Species Reactivity | Human |
| Formulation | 25 µg of lyophilized human IL-18 |
| Research Area | Immunology, Cytokine & Growth Factors |
| Description/Background | Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbial pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells. |
| Storage Temperature | This product is stable for 24 months from the date of manufacture when store at -20°C or below. After reconstitution, avoid repeated freezing and thawing. The IL-18 can be stored for 1 week at 4°C. For storage, prepare appropriate aliquots and freeze them at -80°C using low retention tube. |
| Molecular Weight | 18 kDa |
| Purity | Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining |
| Endotoxin levels | Less than 0.1 ng per 1 µg of recombinant human IL-18 protein, when measured by the LAL assay. |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
B001-5
Cytokine
25 µg