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> SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS]

SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS]

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Cat# GTX635712-100ul

Size : 100ul

Brand : GeneTex

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Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HL455-MS

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus, SARS Coronavirus 2
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX635712 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX635685, Detection: GTX635712
Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888569

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635712

GTX635712 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712

GTX635712 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 (GTX535665), MERS-CoV and SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in ELISA (ELISA). GTX635712

GTX635712 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635712

GTX635712 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] used in Western Blot (WB). GTX635712

GTX635712 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Application Reference

W Deng et al. Signal Transduct Target Ther 2024; 9 (1) : 98 Infection with SARS-CoV-2 can cause pancreatic impairment
Application : IHC-P
S Clever et al. Viruses 2024; 16 (3) : 417 Single MVA-SARS-2-ST/N Vaccination Rapidly Protects K18-hACE2 Mice against a Lethal SARS-CoV-2 Challenge Infection
Application : ELISA
Streblow DN et al. Nat Commun 2023; 14 (1) : 7062 Aerosol delivery of SARS-CoV-2 human monoclonal antibodies in macaques limits viral replication and lung pathology.
Application : ICC/IF
Wei Liu et al. J Virol 2021; 95 (12) Activation of STING Signaling Pathway Effectively Blocks Human Coronavirus Infection.
Application : WB, ICC/IF

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455-MS] (GTX635712). Be the first to share your experience with this product.
Application Reference
W Deng et al. Signal Transduct Target Ther 2024; 9 (1):98 Infection with SARS-CoV-2 can cause pancreatic impairment
Application : IHC-P
S Clever et al. Viruses 2024; 16 (3):417 Single MVA-SARS-2-ST/N Vaccination Rapidly Protects K18-hACE2 Mice against a Lethal SARS-CoV-2 Challenge Infection
Application : ELISA
Streblow DN et al. Nat Commun 2023; 14 (1):7062 Aerosol delivery of SARS-CoV-2 human monoclonal antibodies in macaques limits viral replication and lung pathology.
Application : ICC/IF
Wei Liu et al. J Virol 2021; 95 (12) Activation of STING Signaling Pathway Effectively Blocks Human Coronavirus Infection.
Application : WB, ICC/IF
SDS
PBS.pdf

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