SynaptoGreen™C4
Cat# 70022
Size : 5x1MG
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
SynaptoGreen™ C4 (Equivalent to FM®1-43)
A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.
Technology
Membrane Potential & Nerve Terminal Dyes
Neuronal Probes and Tools
Protocols
Supporting Documents
Product Description
A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen™ when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. Also see AM1-43 (70024), which is similar to SynaptoGreen™, but with a formaldehyde-fixable amine group.
SynaptoGreen™ also can be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.
- λEx/λEm: 510/625 nm (in MeOH); 480/598 nm (in membranes)
- Red solid soluble in water
- C30H49Br2N3
- MW: 612
Nerve Terminal Dyes and Kits
| Product | Catalog No. | Features |
|---|---|---|
| SynaptoGreen™ Dyes (Ex/Em ~480/660 nm in membranes) | 70020... 70053 | View full list of SynaptoGreen™ Dyes |
| SynaptoRed Dyes™ (Ex/Em ~510/750 nm in membranes) | 70021... 70050 | View full list of SynaptoRed™ Dyes |
| Background Reducers | ||
| ADVASEP-7 | 70029 | Sulfonated cyclodextrin that aids in removal of free dye during washes |
| SCAS | 70037 | Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7 |
| Sulforhodamine 101 | 80101 | Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes |
| Nerve Terminal Staining Kits | ||
| Nerve Terminal Staining Kit I | 70030 | Includes SynaptoGreen™ C4 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (A) | 70031 | Includes AM1-43 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (B) | 70031-1 | Includes AM1-43 and SCAS |
| Nerve Terminal Staining Kit III | 70032 | Includes SynaptoGreen™ C4 and Sulforhodamine 101 |
| Nerve Terminal Staining Kit V | 70034 | Includes SynaptoRed™ C2 and ADVASEP-7 |
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.
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