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Reagents and instruments for immunology, cell biology and molecular biology.

Thalidomide [50-35-1]

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Cat# A4216-1ml

Size : 10mM(in1mLDMSO)

Contact local distributor :

Phone : +1 850 650 7790

Brand : APExBIO Technology

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Contact local distributor :

Phone : +1 850 650 7790

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Background

Thalidomide was introduced as a sedative drug,immunomodulatory agent and also is investigated for treating symptoms of many cancers.Thalidomide inhibits an E3 ubiquitin ligase， which is a CRBN-DDB1-Cul4A complex.

Chemical Properties

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	258.23
Cas No.	50-35-1
Formula	C₁₃H₁₀N₂O₄
Solubility	insoluble in H2O; insoluble in EtOH; ≥11.8 mg/mL in DMSO
Chemical Name	2-(2,6-dioxopiperidin-3-yl)isoindole-1,3-dione
SDF	Download SDF
Canonical SMILES	C1CC(=O)NC(=O)C1N2C(=O)C3=CC=CC=C3C2=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:
Cell lines	PBMCs
Preparation method	The solubility of this compound in DMSO is > 11.8 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.
Reacting condition	0 ~ 100 μg/mL
Applications	Thalidomide dose-dependently increased the proliferative responses of PBMCs stimulated by immobilized anti-CD3. As the concentration of anti-CD3 increased, Thalidomide at a constant concentration of 10 μg/mL significantly induced the proliferative responses of PBMCs. However, Thalidomide exhibited no effect on the proliferative response in the absence of anti-CD3. These results indicated that Thalidomide was not mitogenic, Instead, it acted as a costimulator.
Animal experiment [2]:
Animal models	Rabbits
Dosage form	200 mg/kg; p.o.
Applications	In rabbits, Thalidomide at the dose of 200 mg/kg inhibited the area of vascularized cornea, with a median inhibition of 36%. The inhibition of Thalidomide on angiogenesis was seen after only two doses. In addition, the rabbits did not show significant sedation, and there were no signs of toxicity or weight loss.
Other notes	Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References: [1]. Haslett PA, Corral LG, Albert M, Kaplan G. Thalidomide costimulates primary human T lymphocytes, preferentially inducing proliferation, cytokine production, and cytotoxic responses in the CD8+ subset. J Exp Med. 1998 Jun 1;187(11):1885-92. [2]. D'Amato RJ, Loughnan MS, Flynn E, Folkman J. Thalidomide is an inhibitor of angiogenesis. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):4082-5.

Quality Control

Purity = 99.21%

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