Chemotaxis

Chemotaxis

 

Our Chemotaxis Assays use a Boyden Chamber containing a membrane with a defined pore size. Cells are seeded above the membrane and are allowed to migrate toward a chemoattractant in the lower chamber.
 
Advantages :
  • Fully quantify chemotaxis with no manual cell counting
  • Measure chemotaxis in less than 6 hours with most cell types
  • Membrane inserts are uncoated to allow use with any chemoattractant
  • Colorimetric or fluorometric detection
 
For optimal results the membrane pore size should be somewhat smaller than the size of the cell to be assayed. Use the following table to select the right pore size for your migration experiments.

 

 
 
Chemotaxis Assay Selection by Cell Type
 
Cell type
Pore Size
Cell type
Pore size
Astrocytes
12 µm
Lymphocytes
5 µm
Cancer cell lines
8 µm
Macrophages
5 µm
Endothelial cells
8 µm
Monocytes
5 µm
Epithelial cells
8 µm
Neutrophils
3 µm
Fibroblasts
8 µm
Slow-moving cells
12 µm
Leukocytes
3 µm
Tumor cells
8 µm

 

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  • Colorimetry 5
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Description
Cond.
Price Bef. VAT
CBA-125-5
 5x24assays 
CBA-126-5
 5x96assays 
CBA-5037-5
 5x48assays