Ion exchange (IEX) FPLC columns - Positively charged proteins
HiFliQ columns pre-packed and ready to use with sulphonic acid propyl linked (S) coupled Agarose resin for rapid Ion Exchange (IEX) purification of positively charged proteins under native conditions.
Available in 1 ml and 5 ml HiFliQ column sizes with high ligand density and high binding capacity. Compatible with all common chromatography HPLC and FPLC instruments (including ÄKTA FPLC's), and low pressure pumps and syringes using an appropriate adaptor.
Specification :
- Column volume : 1 ml or 5 ml
- Resin : Sulphonic acid propyl linked agarose
- Typical binding capacity : 50-70 mg for 1 ml ; 250-300 mg for 5 ml
- Base matrix : 6% cross-linked agarose
- Mean bead size : 90 µm
- Recommended flow rate : 1 ml/min for 1ml ; 1-6 ml/min for 5 ml
- Max. operating pressure : 0.5 MPa (72 psi)
- External dimensions : 15 mm D. x 80 mm H. for 1 ml ; 23 mm D. x 80 mm H. for 5 ml
- Column construction : Polypropylene
- Inlet Port : 10-32 (1/16") Female
- Outlet Port : 10-32 (1/16") Male
- Storage (2-30°C) : 20% ethanol + 0.2M sodium acetate
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