Antigen-antibody reaction enhancing reagents are designed to improve signal strength and reduce background noise in immunoassay systems such as western blotting and ELISA. By simply diluting antibodies with Signal Boosters, researchers can achieve better results.
Features
- Higher Signals: Possible to obtain 2-10 times higher signals compared to conventional methods using surfactant-containing buffers.
- Wide Compatibility: Used in various immunoassay systems involving antigen-antibody reactions such as western blotting and ELISA.
- Enzyme Compatibility: HRP or ALP is unaffected by Signal Booster. Luminescence or fluorescence detection systems are also compatible.
- Efficiency: Useful for saving antibodies and shortening reaction times.
- Storage: Can be stored in a refrigerator for more than 1 year.
Signal Booster Variants
- Signal Booster Neo: A one-solution system, protein-free, and chemically defined. Ideal for phospho-protein detection or any ELISA, as the protein-free composition avoids interference by proteins in the reagent.
- Signal Booster: A two-component system. Dilute the primary antibody with Solution A and the secondary antibody with Solution B. This version contains protein.
Principle
The main components of the Signal Booster series are polymers and blocking ingredients. The polymer alters the surface physicochemical properties of antigens and antibodies, increasing their contact frequency and specific binding. The combination of polymers and blocking ingredients enhances the signal while reducing background noise.