Akt, phosphorylated (Ser473) (AKT1, Proto-oncogene c-Akt, MGC99656, Protein Kinase B, PKB, PKB-alpha, PRKBA, RAC, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha) (PE)

Akt, phosphorylated (Ser473) (AKT1, Proto-oncogene c-Akt, MGC99656, Protein Kinase B, PKB, PKB-alpha, PRKBA, RAC, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha) (PE)

Cat# A1124-02Q-100ul

Size : 100ul

Brand : US Biological

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A1124-02Q Akt, phosphorylated (Ser473) (AKT1, Proto-oncogene c-Akt, MGC99656, Protein Kinase B, PKB, PKB-alpha, PRKBA, RAC, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha) (PE)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
P31749
Isotype
IgG
Grade
Purified
Applications
FC
Crossreactivity
Bo Ce Ca Ch Eq Hm Hu Mk Mo Po Rb Xe Ze
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3a and b (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3b mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19).||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution: |Flow Cytometry: 1:50|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG|Clone No: 11C1015(D9E)|Host: rabbit|Source: human|Concentration: Not Determined |Form: Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).|Purity: Purified|Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt.|Specificity: Recognizes human Akt, phosphorylated at (Ser473). Species Crossreactivity: mouse, rat, hamster, monkey, mink, chicken, D. melanogaster, xenopus, zebrafish, bovine, canine, porcine, S. cerevisiae, Ce C. elegans and equine||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt.
Form
Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified
Specificity
Recognizes human Akt, phosphorylated at (Ser473). Species Crossreactivity: mouse, rat, hamster, monkey, mink, chicken, D. melanogaster, xenopus, zebrafish, bovine, canine, porcine, S. cerevisiae, Ce C. elegans and equine
References
1) Franke, T.F. et al. (1997) Cell 88, 435-7. (2) Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599- 602. (3) Franke, T.F. et al. (1995) Cell 81, 727-36. (4) Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.|(5) Sarbassov, D.D. et al. (2005) Science 307, 1098-101. (6) Jacinto, E. et al. (2006) Cell 127, 125-37.|(7) Cardone, M.H. et al. (1998) Science 282, 1318-21. (8) Brunet, A. et al. (1999) Cell 96, 857-68.|(9) Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4. (10) Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. (11) Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.|(12) Hajduch, E. et al. (2001) FEBS Lett 492, 199-203. (13) Cross, D.A. et al. (1995) Nature 378, 785-9. (14) Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. (15) Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30. (16) Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52. (17) Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. (18) Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. (19) Manning, B.D. et al. (2002) Mol Cell 10, 151-62.