Akt, phosphorylated (Ser473) (AKT1, Proto-oncogene c-Akt, MGC99656, Protein Kinase B, PKB, PKB-alpha, PRKBA, RAC, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha) (PE)
Cat# A1124-02Q-100ul
Size : 100ul
Brand : US Biological
A1124-02Q Akt, phosphorylated (Ser473) (AKT1, Proto-oncogene c-Akt, MGC99656, Protein Kinase B, PKB, PKB-alpha, PRKBA, RAC, RAC-alpha Serine/threonine-protein Kinase, RAC-PK-alpha) (PE)
Clone Type
PolyclonalHost
rabbitSource
humanSwiss Prot
P31749Isotype
IgGGrade
PurifiedApplications
FCCrossreactivity
Bo Ce Ca Ch Eq Hm Hu Mk Mo Po Rb Xe ZeShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeAkt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3a and b (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3b mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19).||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution: |Flow Cytometry: 1:50|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.