Fig.01 Immunofluorescence of FOXP3 (red) and CD112R (green), magnification 4xFig.02 Immunofluorescence of FOXP3 (red) and CD112R (green), magnification 40xFig.03 FOXP3-IHC in Stroma Colon CaFig.04 IHC detection of FOXP3-positive TILs in Ovarian Ca, (40x)Fig.05 IHC detection of FOXP3-positive TILs in pancreatic Ca (40x)Fig.06 IHC detection of FOXP3-positive TILs in in stomach Ca (20x)Fig.07 IHC detection of FOXP3-positive TILs in stomach Ca (40x)Fig.08 FOXP3-expression in Tonsil (5x)
  • SKU DIA-FX3
    Specificity

    FOXP3
    Species Reactivity

    Human
    Immunogen

    Synthetic peptide of human FOXP3
    Host Species

    Mouse
    Isotype

    IgG2a/k
    Clone

    FX3
    Clonality (Mono-/Polyclonal)

    monoclonal
    Application

    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections), Western Blot
    Conjugation

    unconjugated
    Dilution

    Immunohistochemistry (IHC): 1:100 – 1:200
    Format

    0.05% NaN3, 2% BSA, in PBS (pH 7.4), lyophilisate, purified antibody (from culture supernatant)
    Product line / Topic

    Immuno-Oncology
    Intended Use

    for Research Use Only
    Temperature - Storage

    2-8°C
    Temperature - Transport

    at room temperature
    Search Code

    FOXP3, IHC, FFPE, Paraffin
    Manufacturer / Brand

    ONCOdianova
    Uniprot_ID

    Q9BZS1
    Gene_ID

    50943
    Alias

    AIID, DIETER, Enteropathy, Forkhead Box Protein P3, FOX-P3, FOXP3, Immune Dysregulation, IPEX, JM2, PIDX, Polyendocrinopathy, SCURFIN, X-Linked, XPID
  • Anti-FOXP3 antibody clone FX3 validated for fluorescence multiplex immunohistochemistry

    Clone FX3 has been developed for the detection of FOXP3 in routine formalin-fixed paraffin-embedded tissue specimen (IHC FFPE) and moreover, it has been validated for fluorescence multiplex IHC studies of FOXP3 expression in human tissues.

    FOXP3 (Forkhead box protein P3) is mainly expressed in Regulatory T (Treg) cells. Treg cells are a subset of CD4+ T-cells and play a suppressive role in the immune system. Treg cells ensure immune homeostasis through their ability to suppress the activation and function of leukocytes. Therefore, FOXP3 has emerged as a prominent target for the development of new immunotherapies for cancer and autoimmune diseases.

    The transcription factor FOXP3 acts either as a transcriptional repressor or as a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. FOXP3 is important for the development and inhibitory function of regulatory T-cells (Treg) and coordinates the suppressive activity of Treg cells by activation of different genes, e.g. CTLA4 and TNFRSF18 and by repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG).

  • Fig.01 Immunofluorescence of FOXP3 (red) and CD112R (green), magnification 4x
    Fig. 01: Multicolor Immunofluorescence of FOXP3 (red) and CD112R (green), magnification 4x, normal tonsil.
    Fig.02 Immunofluorescence of FOXP3 (red) and CD112R (green), magnification 40x
    Fig. 02: Multicolor Immunofluorescence of FOXP3 (red) and CD112R (green), magnification 40x, normal tonsil.
    Fig.03 FOXP3-IHC in Stroma Colon Ca
    Fig. 03: FOXP3 positive regulatory T-cells in the Stroma of a colorectal adenocarcinoma.
    Fig.04 IHC detection of FOXP3-positive TILs in Ovarian Ca, (40x)
    Fig. 04: FOXP3 positive TILs in a high grade serous Ovarian carcinoma.
    Fig.05 IHC detection of FOXP3-positive TILs in pancreatic Ca (40x)
    Fig. 05: Focal accumulation of FOXP3 positive TILs in a solid cancer.
    Fig.06 IHC detection of FOXP3-positive TILs in in stomach Ca (20x)
    Fig. 06: Scattered FOXP3 positive TILs in an adenocarcinoma of the stomach.
    Fig.07 IHC detection of FOXP3-positive TILs in stomach Ca (40x)
    Fig. 07: Higher magnification of the same cancer as in Figure 6. Note the optimal signal to noise ratio of immunostaining.
    Fig.08 FOXP3-expression in Tonsil (5x)
    Fig. 08: Normal distribution of FOXP3 positive cells in a tonsil.