Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Positive for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Negative for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3 K27M mutant, stained with 0.01 µg/mL of anti-Histone H3 K27M rabbit monoclonal antibody, Clone RM192.
Product Details Product Name Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192 Product Description Rabbit monoclonal to Histone H3 K27M; Histone H3, K27M Mutant Catalog No. 31-1175-00-S;
31-1175-00-L;
31-1175-02 Clone Name RM192 Product Data Sheet Anti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192
Biotinylated Anti-Histone H3 K27M Rabbit Monoclonal Antibody Data Sheet RM192 Specificity RM192 reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3. Immunogen A peptide corresponding to Histone H3 K27M mutant Application Western Blot
ELISA
Immunohistochemistry
Species Reactivity All Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 1:250 – 1:2500;
ELISA: 1:250 – 1:2500;
IHC: 1:100 – 1:500. References for Histone H3 K27M Mutant antibody [RM192] Thomas L et al OKlahoma Nitrone-007: novel treatment for diffuse intrinsic pontine glioma. J Transl Med. (2020), 10.1186/s12967-020-02593-5. IHC; Human. Read more (PubMed: 33168005)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Mondal G et al Pediatric bithalamic gliomas have a distinct epigenetic signature and frequent EGFR exon 20 insertions resulting in potential sensitivity to targeted kinase inhibition. Acta Neuropathol. (2020), 10.1007/s00401-020-02155-5. IHC; Human. Read more (PubMed: 32303840)
Maeda S et al H3F3A mutant allele specific imbalance in an aggressive subtype of diffuse midline glioma, H3 K27M-mutant. Acta Neuropathol Commun. (2020), 10.1186/s40478-020-0882-4. WB, IHC; Human. Read more (PubMed: 32019606)
Chiang J et al Clinical, imaging, and molecular analysis of pediatric pontine tumors lacking characteristic imaging features of DIPG. Acta Neuropathol Commun. (2020), 10.1186/s40478-020-00930-9. IHC; Human. Read more (PubMed: 32326973)
Tanrıkulu B et al. Is H3K27me3 status really a strong prognostic indicator for pediatric posterior fossa ependymomas? A single surgeon, single center experience. Childs Nerv Syst. (2020), 10.1007/s00381-020-04518-5. IHC-F; Human. Read more (PubMed: 32025869)
Qiu T et al. Imaging characteristics of adult H3 K27M-mutant gliomas. J Neurosurg. (2019), 10.3171/2019.9.JNS191920. IHC; Human. Read more (PubMed: 31731269)
Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533) . Product Images
Anti-Histone H3 K27M rabbit monoclonal antibody [RM192]
![Anti-Histone H3 K27M rabbit monoclonal antibody [RM192]](https://www.clinisciences.com/en/upload/-img-lapin1.png)
Cat# 31-1175-00-S
Size : 100ul
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Histone H3 K27M;
Histone H3, K27M Mutant;
100 µL Small Size, 400 µL Large Size, 50 µL Biotin Conjugate
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