RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3. Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 µg/mL, showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells. Immunohistochemical staining of formalin fixed and paraffin embedded human seminoma tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175. Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175. Immunohistochemical staining of formalin fixed and paraffin embedded human glioblastoma tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175. Immunohistochemical staining of formalin fixed and paraffin embedded human tumor tissue section using Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175, from user review.
Product Details Product Name Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175 Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27) Catalog No. 31-1062-00 Clone Name RM175 Product Data Sheet Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175 Specificity RM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3. Immunogen A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27). Application Chromatin IP (ChIP)
Western Blot (WB)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ChIP: 1 µg - 5 µg;
Western Blot: 1 µg/mL – 2 µg/mL;
IHC: 0.5 µg/mL – 2.5 µg/mL;
ELISA: 0.5 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL. . Product Images References for Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27) [RM175] Andrea J Kriz et al. Balancing cohesin eviction and retention prevents aberrant chromosomal interactions, Polycomb-mediated repression, and X-inactivation Mol Cell (IF: 17.97; Q1)
. 2021 May 6;81(9):1970-1987. ChIP; Read more (PMID: 33725485)
Chia-Chi Tsai et al. Overexpression of Histone H3 Lysine 27 Trimethylation Is Associated with Aggressiveness and Dedifferentiation of Thyroid Cancer Endocr Pathol 2019 Dec;30(4):305-311. Human IHC Read more (PMID: 31396854)
Chen-Yu Wang et al. PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments Nat Commun (IF: 14.92; Q1). 2019 Jul 3;10(1):2950. ICC/IF ; Read more (PMID: 31270318)
Peitao Lü et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. Nat Plants (IF: 13.26; Q1). 2018 Oct;4(10):784-791 ChIP; Read more (PMID: 30250279)
Chen-Yu Wang et al. SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X Cell (IF: 41.58; Q1). 2018 Jul 12;174(2):406-421. ICC/IF ; Read more (PMID: 29887375)
Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175]
![Anti-Trimethyl-Histone H3 (Lys27) rabbit monoclonal antibody [RM175]](https://www.clinisciences.com/en/upload/-img-lapin1.png)
Cat# 31-1062-00
Size : 100ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
25 µg Trial Size, 100 µg Full Size
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