Auxinole [86445-22-9]
Cat# HY-111444-10mg
Size : 10mg
Brand : MedChemExpress
Auxinole is a potent auxin antagonist of TIR1/AFB receptors, binding TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and so inhibits auxin-responsive gene expression.
For research use only. We do not sell to patients.
Auxinole Chemical Structure
CAS No. : 86445-22-9
This product is a controlled substance and not for sale in your territory.
Based on 12 publication(s) in Google Scholar
Description |
Auxinole is a potent auxin antagonist of TIR1/AFB receptors, binding TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and so inhibits auxin-responsive gene expression. |
IC50 & Target |
TIR1/AFB receptor[1] |
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In Vitro |
Auxinole is a potent auxin antagonist of TIR1/AFB receptors, and binds TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and then inhibits auxin-responsive gene expression. In addition, Auxinole competitively inhibits various auxin responses in planta[1]. Auxinole causes reduction in IAA-triggered depolarization in root hair cells. Auxinole (20 μM) also represses the transient increase in [Ca2+]cyt completely, and blocks Ca2+ response[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
321.37 |
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Formula |
C20H19NO3 |
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CAS No. |
86445-22-9 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=C(O)C(CC(C1=CC=C(C)C=C1C)=O)C2=CNC3=C2C=CC=C3 |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
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Solvent & Solubility |
In Vitro:
DMSO : ≥ 125 mg/mL (388.96 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Ethanol : 2.5 mg/mL (7.78 mM; ultrasonic and warming and heat to 60°C) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.77% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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Ethanol / DMSO | 1 mM | 3.1117 mL | 15.5584 mL | 31.1168 mL | 77.7920 mL |
5 mM | 0.6223 mL | 3.1117 mL | 6.2234 mL | 15.5584 mL | |
DMSO | 10 mM | 0.3112 mL | 1.5558 mL | 3.1117 mL | 7.7792 mL |
15 mM | 0.2074 mL | 1.0372 mL | 2.0745 mL | 5.1861 mL | |
20 mM | 0.1556 mL | 0.7779 mL | 1.5558 mL | 3.8896 mL | |
25 mM | 0.1245 mL | 0.6223 mL | 1.2447 mL | 3.1117 mL | |
30 mM | 0.1037 mL | 0.5186 mL | 1.0372 mL | 2.5931 mL | |
40 mM | 0.0778 mL | 0.3890 mL | 0.7779 mL | 1.9448 mL | |
50 mM | 0.0622 mL | 0.3112 mL | 0.6223 mL | 1.5558 mL | |
60 mM | 0.0519 mL | 0.2593 mL | 0.5186 mL | 1.2965 mL | |
80 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9724 mL | |
100 mM | 0.0311 mL | 0.1556 mL | 0.3112 mL | 0.7779 mL |
Auxinole Related Classifications
- Others
- Others