Catalase (CAT) BioAssay™ ELISA Kit (Human)

The Catalase (CAT) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CAT in human serum, plasma, cell lysates, tissue homogenates, cell culture supernatants and other biological fluids.||Detection Range:|0.312-20ng/ml||Sensitivity:|<0.113ng/ml||Precision:|Intra-Assay: CV<10%|Inter-Assay: CV<12%||Kit Components:|*023986A: Microtiter Plate, 1x96 wells. Pre-coated; ready to use.|*023986B: Standard, 2x1vial |023986C: Standard Diluent, 1x20ml|*023986D: Detection Reagent A, 1x120ul |*023986E: Detection Reagent B, 1x120ul |023986F: Assay Diluent A, 1x12ml|023986G: Assay Diluent B, 1x12ml|023986H: TMB Substrate, 1x9ml|023986K: Stop Solution, 1x6ml|023986L: Wash Buffer, 30X, 1x20ml||Storage and Stability:|Store *023986A, *023986B, *023986D and *023986E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 100ul standard or sample to each well. Incubate for 1 hour at 37°C.|3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediately.