CD4 antibody [SK3] (PerCP-Cy5.5)
Cat# GTX01461-11-100test
Size : 100test
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-CD4 antibody [SK3] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01461-11](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD4 antibody [SK3] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX01461-11")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | SK3 |
Isotype | IgG1 |
Application | FACS |
Reactivity | Human, Cynomolgus monkey, Rhesus Monkey |
| Package | 100 test |
Datasheet File View product citations for antibody GTX01461-11 on CiteAb
PRODUCT
Summary
The SK3 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The SK3 antibody is widely used as a phenotypic marker for human CD4 expression, and has been reported to be cross-reactive with Rhesus and Cynomolgus CD4. This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | 0.06 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.012 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD4 molecule , CD4 , CD4mut
Cellular Localization
Cell membrane
Background
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
Database
DATA IMAGES
| | GTX01461-11 FACS Image FACS analysis of human peripheral blood lymphocytes using GTX01461-11 CD4 antibody [SK3] (PerCP-Cy5.5). |
Clone Reference
2012; IL-7 abrogates suppressive activity of human CD4+CD25+FOXP3+ regulatory T cells and allows expansion of alloreactive and autoreactive T cells.
2000; One-round determination of seven leukocyte subsets in rhesus macaque blood by flow cytometry.
2000; Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
1986; Epitopes of the CD4 antigen and HIV infection.
1981; Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen.
REVIEW
Clone Reference
2012; IL-7 abrogates suppressive activity of human CD4+CD25+FOXP3+ regulatory T cells and allows expansion of alloreactive and autoreactive T cells.
2000; One-round determination of seven leukocyte subsets in rhesus macaque blood by flow cytometry.
2000; Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
1986; Epitopes of the CD4 antigen and HIV infection.
1981; Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen.
| SDS | |
|---|---|
| Sodium Azide.pdf | |