Images
Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.
Note

Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.

Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.

(A) HL-60 cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite beta-D-galactopyranoside for 30 mins at 37°C. The signal was acquired using FITC channel in ACEA NovoCyte flow cytometer.
(B) HeLa cells were incubated with Camptothecin for 6 hours to induce senescence and stained with Xite beta-D-galactopyranoside for 30 mins at 37°C. The signal was acquired using FITC filter set.

  • Specifications

    Product Description

    Cellular Senescence Activity Assay Kit uses Xite beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by SA-beta-gal inside cells, generating strong green fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. The Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity with robust performance.

    Suitable Sample

    Cells.

    Detection Method

    Fluorimetric

    Platform

    Instrument: Flow cytometer
    Excitation: 488 nm laser
    Emission: 530/30 nm filter
    Instrument specification(s): FITC channel

    Instrument: Fluorescence microscope
    Excitation: FITC filter set
    Emission: FITC filter set
    Recommended plate: Black wall/clear bottom

    Regulatory Status

    For research use only (RUO)

    Storage Instruction

    Store the kit at -20°C and avoid from light.

    Note

    Cellular senescence was measured with the Cellular Senescence Activity Assay Kit using a (A) NovoCyte Flow Cytometer (ACEA Biosciences) and (B) fluorescence microscope.

  • Applications

    Functional Study

    Flow Cytometry