Goat Anti-Human IgG-AF647
Cat# 2040-31
Size : 1.0mg
Brand : Southern Biotech
Contact local distributor :
Phone : +1 850 650 7790
| Isotype | Goat IgG |
|---|---|
| Isotype Control | Goat IgG-AF647 |
| Specificity | Reacts with the heavy chain of human IgG |
| Source | Pooled antisera from goats hyperimmunized with human IgG |
| Cross Adsorption | Human IgM and IgA; may react with IgG from other species |
| Purification Method | Affinity chromatography on human IgG covalently linked to agarose |
| Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Polyclonal |
| Concentration | 1.0 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Quality tested applications for relevant formats include - ELISA 2-5 FLISA 6 Flow Cytometry 1,10,13,14 Other referenced applications for relevant formats include - ELISpot 2,3,11 Immunohistochemistry-Frozen Sections 7,8 Immunohistochemistry-Paraffin Sections 9 Immunocytochemistry 10-12 Western Blot 4,12,17,18 Immunoprecipitation 19 Multiplex 3,15,16 Depletion 14,20 |
| RRID Number | AB_2795651 |
Documentation
Technical Bulletin Safety Datasheet
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- 2. Traggiai E, Volpi S, Schena F, Gattorno M, Ferlito F, Moretta L, et al. Bone marrow-derived mesenchymal stem cells induce both polyclonal expansion and differentiation of B cells isolated from healthy donors and systemic lupus erythematosus patients. Stem Cells. 2008;26:562-9. (ELISA, ELISPOT)
- 3. Staats HF, Kirwan SM, Whisnant CC, Stephenson JL, Wagener DK, Majumder PP. Development of a bead immunoassay to measure Vi polysaccharide-specific serum IgG after vaccination with the Salmonella enterica serovar Typhi Vi polysaccharide. Clin Vaccine Immunol. 2010;17:412-9. (ELISA, Multiplex)
- 4. Lai H, Engle M, Fuchs A, Keller T, Johnson S, Gorlatov S, et al. Monoclonal antibody produced in plants efficiently treats West Nile virus infection in mice. Proc Natl Acad Sci USA. 2010;107:2419-24. (ELISA, WB)
- 5. Kwa S, Lai L, Gangadhara S, Siddiqui M, Pillai VB, Labranche C, et al. CD40L-adjuvanted DNA/modified vaccinia virus Ankara simian immunodeficiency virus SIV239 vaccine enhances SIV-specific humoral and cellular immunity and improves protection against a heterologous SIVE660 mucosal challenge. J Virol. 2014;88:9579-89. (ELISA)
- 6. Spensieri F, Borgogni E, Zedda L, Bardelli M, Buricchi F, Volpini G, et al. Human circulating influenza-CD4+ ICOS1+IL-21+ T cells expand after vaccination, exert helper function, and predict antibody responses. Proc Natl Acad Sci USA. 2013;110:14330-5. (FLISA)
- 7. Borrego L, Maynard B, Peterson EA, George T, Iglesias L, Peters MS, et al. Deposition of eosinophil granule proteins precedes blister formation in bullous pemphigoid. Comparison with neutrophil and mast cell granule proteins. Am J Pathol. 1996;148:897-909. (IHC-FS)
- 8. Forshammar J, Isaksson S, Strid H, Stotzer P, Sjövall H, Simrén M, et al. A pilot study of colonic B cell pattern in irritable bowel syndrome. Scand J Gastroenterol. 2008;43:1461-6. (IHC-FS)
- 9. Rowley AH, Shulman ST, Mask CA, Finn LS, Terai M, Baker SC, et al. IgA plasma cell infiltration of proximal respiratory tract, pancreas, kidney, and coronary artery in acute Kawasaki disease. J Infect Dis. 2000;182:1183-91. (IHC-PS)
- 10. Oritani K, Kincade PW. Identification of stromal cell products that interact with pre-B cells. J Cell Biol. 1996;134:771-82. (ICC, FC)
- 11. Alwayn IP, Xu Y, Basker M, Wu C, Buhler L, Lambrigts D, et al. Effects of specific anti-B and/or anti-plasma cell immunotherapy on antibody production in baboons: depletion of CD20- and CD22-positive B cells does not result in significantly decreased production of anti-αGal antibody. Xenotransplantation. 2001;8:157-71. (ICC, ELISPOT)
- 12. Hasegawa H, Forte C, Barber I, Turnbaugh S, Stoops J, Shen M, et al. Modulation of in vivo IgG crystallization in the secretory pathway by heavy chain isotype class switching and N-linked glycosylation. Biochim Biophys Acta. 2014;1843:1325-38. (ICC, WB)
- 13. Kudo K, Imai C, Lorenzini P, Kamiya T, Kono K, Davidoff AM, et al. T lymphocytes expressing a CD16 signaling receptor exert antibody-dependent cancer cell killing. Cancer Res. 2014;74:93-103. (FC)
- 14. Dryer RL, Covey LR. Use of chromatin immunoprecipitation (ChIP) to detect transcription factor binding to highly homologous promoters in chromatin isolated from unstimulated and activated primary human B cells. Biol Proced Online. 2006;8:44-54. (FC, Depletion)
- 15. Pochechueva T, Chinarev A, Bovin N, Fedier A, Jacob F, Heinzelmann-Schwarz V. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. J Immunol Methods. 2014;412:42-52. (Multiplex)
- 16. Pickering JW, Larson MT, Martins TB, Copple SS, Hill HR. Elimination of false-positive results in a luminex assay for pneumococcal antibodies. Clin Vaccine Immunol. 2010;17:185-9. (Multiplex)
- 17. Tran M, Zhou B, Pettersson PL, Gonzalez MJ, Mayfield SP. Synthesis and assembly of a full-length human monoclonal antibody in algal chloroplasts. Biotechnol Bioeng. 2009;104:663-73. (WB)
- 18. Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (WB)
- 19. Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, et al. FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol. 2011;23:43-53. (IP)
- 20. Palaia JM, McConnell M, Achenbach JE, Gustafson CE, Stoermer KA, Nolan M, et al. Neutralization of HIV subtypes A and D by breast milk IgG from women with HIV infection in Uganda. J Infect. 2014;68:264-72. (Depletion)