Goat Anti-Mouse IgG2b, Human ads-BIOT

Cat# 1090-08

Size : 1.0mg

Brand : Southern Biotech

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Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy chain of mouse IgG2b
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Cross Adsorption Mouse IgG1, IgG2a, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2b covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-12
FLISA
Flow Cytometry 13-15

Other referenced applications for relevant formats include -
ELISpot 4,6,16
Immunohistochemistry-Frozen Sections 17-19
Immunohistochemistry-Paraffin Sections 17,20
Immunocytochemistry 21,22
Western Blot 23,24
RRID Number AB_2794523
Gene ID 16016 (Mouse)
Gene ID Symbol Ighg2b (Mouse)
Gene ID Aliases IgG2b; Igh-3; gamma2b
UniProt ID P01867 (Mouse
UniProt Name IGG2B_MOUSE (Mouse)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  2. 2. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)
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  7. 7. Futatsugi-Yumikura S, Matsushita K, Fukuoka A, Takahashi S, Yamamoto N, Yonehara S, et al. Pathogenic Th2-type follicular helper T cells contribute to the development of lupus in Fas-deficient mice. Int Immunol. 2014;26:221-31. (ELISA)
  8. 8. Aguilar JL, Varshney AK, Wang X, Stanford L, Scharff M, Fries BC. Detection and measurement of staphylococcal enterotoxin-like K (SEl-K) secretion by Staphylococcus aureus clinical isolates. J Clin Microbiol. 2014;52:2536-43. (ELISA)
  9. 9. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA)
  10. 10. Quakkelaar ED, Fransen MF, van Maren WW, Vaneman J, Loof NM, van Heiningen SH, et al. IgG-mediated anaphylaxis to a synthetic long peptide vaccine containing a B cell epitope can be avoided by slow-release formulation. J Immunol. 2014;192:5813-20. (ELISA)
  11. 11. Patel JM, Vartabedian VF, Kim M, He S, Kang S, Selvaraj P. Influenza virus-like particles engineered by protein transfer with tumor-associated antigens induces protective antitumor immunity. Biotechnol Bioeng. 2015;112:1102-10. (ELISA)
  12. 12. Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79. (ELISA)
  13. 13. Gliddon DR, Howard CJ. CD26 is expressed on a restricted subpopulation of dendritic cells in vivo. Eur J Immunol. 2002;32:1472-81. (FC)
  14. 14. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC)
  15. 15. Klausmann S, Sydler T, Summerfield A, Lewis FI, Weilenmann R, Sidler X, et al. T-cell reprogramming through targeted CD4-coreceptor and T-cell receptor expression on maturing thymocytes by latent Circoviridae family member porcine circovirus type 2 cell infections in the thymus. Emerg Microbes Infect. 2015;4:e15. (FC)
  16. 16. Hsu H, Wu Y, Yang P, Wu Q, Job G, Chen J, et al. Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J Immunol. 2007;178:5357-65. (ELISPOT)
  17. 17. van der Loos C. User Protocol: Practical Guide to Multiple Staining. BioTechniques' Protocol Guide 2010. 2009 Nov. doi: doi10.2144/000113281. (IHC-FS, IHC-PS)
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  20. 20. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (IHC-PS)
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  23. 23. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in CH1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
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