Human IgA (alpha chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500.
Reactivities	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Human IgA alpha chain.
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized purified Ig fraction. Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.3 moles TRITC per mole of Rabbit IgG.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	TRITC
Storage	Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.