Human Pancreas cDNA

Cat# 44713cDNA

Size : 50µg

Brand : Celprogen

Contact local distributor :


Phone : +1 850 650 7790

  • Product Description

     Human Pancreas cDNA

    Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 110 ng total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40 µl final volume. RT Reaction stopped by heating at 70°C for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCI, 3 mM MgCl2, 10mM DTT). The estimated cDNA concentration is about 2.5 ng/µl. 1 µl cDNA is good enough for one PCR reaction.

    Source: Adult Human Pancreas

    Storage Conditions: -20°C or colder

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