Reagents and instruments for immunology, cell biology and molecular biology.
 
> Influenza A virus PB2 protein antibody

Influenza A virus PB2 protein antibody

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Cat# GTX125925-100ul

Size : 100ul

Brand : Genetex

Contact local distributor :


Phone : +1 850 650 7790

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125925
Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125925

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX125925 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

WSN-infected DF-1 cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170601

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 2 , Influenza A Virus PB2

DATA IMAGES

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125925

GTX125925 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
7.5% SDS PAGE
GTX125925 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125925

GTX125925 ICC/IF Image

Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125925) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Application Reference

Staller E et al. Nat Commun 2024; 15 (1) : 4123 Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation.
HTW Blest et al. Cell Rep 2024; 43 (5) : 114122 HSV-1 employs UL56 to antagonize expression and function of cGAMP channels
E Fodor et al. Research Square 2024; Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation
Application : WB
Krischuns T et al. Nat Commun 2024; 15 (1) : 1064 The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.
Chen Y et al. Influenza Other Respir Viruses 2023; 17 (3) : e13112 Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza.
Application : WB
Submit a Reference

REVIEW

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Application Reference
Staller E et al. Nat Commun 2024; 15 (1):4123 Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation.
HTW Blest et al. Cell Rep 2024; 43 (5):114122 HSV-1 employs UL56 to antagonize expression and function of cGAMP channels
E Fodor et al. Research Square 2024; Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation
Application : WB
Krischuns T et al. Nat Commun 2024; 15 (1):1064 The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.
Chen Y et al. Influenza Other Respir Viruses 2023; 17 (3):e13112 Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza.
Application : WB
Jianyuan Zhao et al. Nat Commun 2022; 13 (1):2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.
Application : WB
Masami Shiimori et al. iScience 2021; 24 (6):102660 Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation.
Application : WB
Sun N et al. Cell Rep 2020; 33 (5):108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.
Application : WB
Chen KY et al. PLoS Pathog 2019; 15 (10):e1008034 Influenza virus polymerase subunits co-evolve to ensure proper levels of dimerization of the heterotrimer.
Application : WB
Su WC et al. J Virol 2015; 89 (22):11245-55 A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

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