KIAA0319L (NM_024874) Human Tagged ORF Clone

CAT#: RC202618

  • TrueORF®

KIAA0319L (Myc-DDK-tagged)-Human KIAA0319-like (KIAA0319L)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KIAA0319L
Synonyms AAVR; AAVRL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202618 representing NM_024874
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGAGGCTGGGAGTCAAGCCAAATCCTGCTTCCTGGATTTTATCAGGATATTATTGGCAGACAT
CTGCGAAGTGGTTGAGAAGCCTGTACCTGTTTTATACTTGCTTTTGCTTCAGCGTTCTGTGGTTGTCAAC
AGATGCCAGTGAGAGCAGGTGCCAGCAGGGGAAGACACAATTTGGAGTTGGCCTGAGATCTGGGGGAGAA
AATCACCTCTGGCTTCTTGAAGGAACCCCCTCTCTCCAGTCATGTTGGGCTGCCTGCTGCCAGGACTCTG
CCTGCCATGTCTTTTGGTGGCTAGAAGGGATGTGCATTCAGGCAGACTGCAGCAGGCCCCAGAGCTGCCG
GGCTTTTAGGACACACTCCTCCAATTCCATGCTGGTGTTTTTAAAAAAATTCCAAACTGCAGATGATTTG
GGCTTTCTACCTGAAGATGATGTACCACATCTTCTGGGGCTAGGTTGGAACTGGGCATCTTGGAGGCAGA
GCCCACCCAGAGCTGCACTCAGACCTGCTGTATCTTCCAGTGACCAGCAGAGCTTAATCAGGAAGCTTCA
GAAGAGAGGTAGTCCCAGTGACGTAGTTACACCTATAGTGACACAGCATTCTAAAGTGAATGACTCCAAC
GAATTAGGTGGTCTGACTACCAGTGGCTCTGCAGAGGTCCACAAGGCGATTACAATTTCCAGTCCCCTAA
CCACAGACCTGACTGCAGAGCTGTCTGGTGGGCCAAAGAATGTATCAGTGCAACCTGAAATATCAGAGGG
TCTTGCTACTACGCCCAGCACTCAACAAGTAAAAAGTTCTGAGAAAACCCAGATTGCTGTCCCCCAGCCA
GTGGCTCCCTCCTACAGTTATGCTACCCCTACCCCCCAGGCCTCTTTCCAGAGCACCTCAGCACCATACC
CAGTTATAAAGGAACTGGTGGTATCTGCTGGAGAGAGTGTCCAGATAACCCTGCCTAAGAATGAAGTTCA
ATTAAATGCATATGTTCTCCAAGAACCACCTAAAGGAGAAACCTACACCTACGACTGGCAGCTGATTACT
CATCCTAGAGACTACAGTGGAGAAATGGAAGGGAAACATTCCCAGATCCTCAAACTATCGAAGCTCACTC
CAGGCCTGTATGAATTCAAAGTGATTGTAGAGGGTCAAAATGCCCATGGGGAAGGCTATGTGAACGTGAC
AGTCAAGCCAGAGCCCCGTAAGAATCGGCCCCCCATTGCTATTGTGTCACCTCAGTTCCAGGAGATCTCT
TTGCCAACCACTTCTACAGTCATTGATGGCAGTCAAAGCACTGATGATGATAAAATCGTTCAGTACCATT
GGGAAGAACTTAAGGGGCCTCTAAGAGAAGAGAAGATTTCTGAAGATACAGCCATATTAAAACTAAGTAA
ACTCGTCCCTGGGAACTACACTTTCAGCTTGACTGTAGTAGACTCTGATGGAGCTACCAACTCTACTACT
GCAAACCTGACAGTGAACAAAGCTGTGGATTACCCCCCTGTGGCCAACGCAGGCCCCAACCAAGTGATCA
CCCTGCCCCAAAACTCCATCACCCTCTTTGGGAACCAGAGCACTGATGATCATGGCATCACCAGCTATGA
GTGGTCACTCAGCCCAAGCAGCAAAGGGAAAGTGGTGGAGATGCAGGGTGTTAGAACACCAACCTTACAG
CTCTCTGCGATGCAAGAAGGAGACTACACTTACCAGCTCACAGTGACTGACACAATAGGACAGCAGGCCA
CTGCTCAAGTGACTGTTATTGTGCAACCTGAAAACAATAAGCCTCCTCAGGCAGATGCAGGCCCAGATAA
AGAGCTGACCCTTCCTGTGGATAGCACAACCCTGGATGGCAGCAAGAGCTCAGATGATCAGAAAATTATC
TCATATCTCTGGGAAAAAACACAGGGACCTGATGGGGTGCAGCTCGAGAATGCTAACAGCAGTGTTGCTA
CTGTGACTGGGCTGCAAGTGGGGACCTATGTGTTCACCTTGACTGTCAAAGATGAGAGGAACCTGCAAAG
CCAGAGCTCTGTGAATGTCATTGTCAAAGAAGAAATAAACAAACCACCTATAGCCAAGATAACTGGGAAT
GTGGTGATTACCCTACCCACGAGCACAGCAGAGCTGGATGGCTCTAAGTCCTCAGATGACAAGGGAATAG
TCAGCTACCTCTGGACTCGAGATGAGGGGAGCCCAGCAGCAGGGGAGGTGTTAAATCACTCTGACCATCA
CCCTATCCTTTTTCTTTCAAACCTGGTTGAGGGAACCTACACTTTTCACCTGAAAGTGACCGATGCAAAG
GGTGAGAGTGACACAGACCGGACCACTGTGGAGGTGAAACCTGATCCCAGGAAAAACAACCTGGTGGAGA
TCATCTTGGATATCAACGTCAGTCAGCTAACTGAGAGGCTGAAGGGGATGTTCATCCGCCAGATTGGGGT
CCTCCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGCAGAGCACCAAA
ATGGTATTTTTTGTTCAAAACGAGCCTCCCCACCAGATCTTCAAAGGCCATGAGGTGGCAGCGATGCTCA
AGAGTGAGCTGCGGAAGCAAAAGGCAGACTTTTTGATATTCAGAGCCTTGGAAGTCAACACTGTCACATG
TCAGCTGAACTGTTCCGACCATGGCCACTGTGACTCGTTCACCAAACGCTGTATCTGTGACCCTTTTTGG
ATGGAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAGTGGAGCGTGTTATATGTTA
TCATTGCTACCTTTGTCATTGTTGTTGCCTTGGGAATCCTGTCTTGGACTGTGATCTGTTGTTGTAAGAG
GCAAAAAGGAAAACCCAAGAGGAAAAGCAAGTACAAGATCCTGGATGCCACGGATCAGGAAAGCCTGGAG
CTGAAGCCAACCTCCCGAGCAGGCATCAAACAGAAAGGCCTTTTGCTAAGTAGCAGCCTGATGCACTCCG
AGTCAGAGCTGGACAGCGATGATGCCATCTTTACATGGCCAGACCGAGAGAAGGGCAAACTCCTGCATGG
TCAGAATGGCTCTGTACCCAACGGGCAGACCCCTCTGAAGGCCAGGAGCCCGCGGGAGGAGATCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202618 representing NM_024874
Red=Cloning site Green=Tags(s)

MEKRLGVKPNPASWILSGYYWQTSAKWLRSLYLFYTCFCFSVLWLSTDASESRCQQGKTQFGVGLRSGGE
NHLWLLEGTPSLQSCWAACCQDSACHVFWWLEGMCIQADCSRPQSCRAFRTHSSNSMLVFLKKFQTADDL
GFLPEDDVPHLLGLGWNWASWRQSPPRAALRPAVSSSDQQSLIRKLQKRGSPSDVVTPIVTQHSKVNDSN
ELGGLTTSGSAEVHKAITISSPLTTDLTAELSGGPKNVSVQPEISEGLATTPSTQQVKSSEKTQIAVPQP
VAPSYSYATPTPQASFQSTSAPYPVIKELVVSAGESVQITLPKNEVQLNAYVLQEPPKGETYTYDWQLIT
HPRDYSGEMEGKHSQILKLSKLTPGLYEFKVIVEGQNAHGEGYVNVTVKPEPRKNRPPIAIVSPQFQEIS
LPTTSTVIDGSQSTDDDKIVQYHWEELKGPLREEKISEDTAILKLSKLVPGNYTFSLTVVDSDGATNSTT
ANLTVNKAVDYPPVANAGPNQVITLPQNSITLFGNQSTDDHGITSYEWSLSPSSKGKVVEMQGVRTPTLQ
LSAMQEGDYTYQLTVTDTIGQQATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSSDDQKII
SYLWEKTQGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPIAKITGN
VVITLPTSTAELDGSKSSDDKGIVSYLWTRDEGSPAAGEVLNHSDHHPILFLSNLVEGTYTFHLKVTDAK
GESDTDRTTVEVKPDPRKNNLVEIILDINVSQLTERLKGMFIRQIGVLLGVLDSDIIVQKIQPYTEQSTK
MVFFVQNEPPHQIFKGHEVAAMLKSELRKQKADFLIFRALEVNTVTCQLNCSDHGHCDSFTKRCICDPFW
MENFIKVQLRDGDSNCEWSVLYVIIATFVIVVALGILSWTVICCCKRQKGKPKRKSKYKILDATDQESLE
LKPTSRAGIKQKGLLLSSSLMHSESELDSDDAIFTWPDREKGKLLHGQNGSVPNGQTPLKARSPREEIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024874
ORF Size 3147 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024874.5
RefSeq Size 4822 bp
RefSeq ORF 3150 bp
Locus ID 79932
UniProt ID Q8IZA0
Cytogenetics 1p34.3
Domains PKD
Protein Families Transmembrane
MW 115.7 kDa
Gene Summary This gene is a candidate gene for dyslexia susceptibility.[provided by RefSeq, Apr 2009]

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