MICA Mouse Monoclonal Antibody [Clone ID: BAMO3]
Specifications
Product Data | |
Clone Name | BAMO3 |
Applications | ELISA, FC, IP |
Recommended Dilution | Immunoprecipitation: 2-5 μg/300 μl of cell extract. Flow Cytometry: 10 μg/ml (final concentration). ELISA: 1 μg/ml (for detector antibody). Western blot: Not recommended. For more details See Protocols below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | MICA*01, MICA*04 and MICB*02 transfected P815 cells |
Specificity | This antibody reacts with MICA/B. |
Formulation | PBS containing 50% Glycerol, pH 7.2 State: Azide Free State: Liquid purified IgG fraction from Hybridoma (Clone BAMO3) Supernatant Preservative: None |
Concentration | lot specific |
Purification | Protein A Agarose Chromatography |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | MHC class I polypeptide-related sequence A |
Database Link | |
Background | MICA and MICB (Major Histocompatibility Complex class I Chain-related gene A and gene B) bind to the activating immunoreceptor NKG2D. NKG2D is expressed on NK (Natural Killer) cells, NKT cells, ï§ï¤T cells and CD8+ï¡ï¢T cells. Recognition of MICA and MICB by NKG2D is involved in tumor surveillance, immune responses to viral infections and autoimmune diseases. MICA and MICB are transmembrane glycoproteins that are distantly related to the MIC proteins, and they possess three extra-cellular Ig-like domains. And thus, MICA and MICB are closely related but are functionally indistinguishable. MICA and MICB molecules are highly glycosylated, and are detected as a smear band ranging from 65-75 kDa. It is reported that MICA and MICB are highly expressed in variant tumor cells, whereas normal cells express little. Tumor cells have been shown to shed and release MIC molecules from the cell surface. Therefore determination of soluble MIC (sMIC) levels provides valuable information for cancer staging, and sMIC in serum seems to be an indicator for systemic manifestation of malignancy rather than for local tumor extent. |
Synonyms | MHC class I polypeptide-related sequence A, MIC-A, PERB11.1, MHC class I polypeptide-related sequence B, MIC-B, PERB11.2 |
Note | This product was originally produced by MBL International. Protocol: Immunoprecipitation Flow Cytometric analysis for floating cells |
Reference Data |
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